Neeleman R, Joerink M, Beuvery C, van Boxtel T
National Institute of Public Health and the Environment, Laboratory for Product and Process Development, Bilthoven, The Netherlands.
Appl Microbiol Biotechnol. 2001 Nov;57(4):489-93. doi: 10.1007/s002530100811.
To improve the cultivation of Bordetella pertussis and take advantage of the newest techniques in monitoring and control, a quantitative description of substrate utilisation is necessary. Growth of the organism is limited by two main substrates. However neither interactive nor non-interactive modelling seem appropriate. A model that combines essential and enhanced kinetics was developed based on experimental observation. Instead of fitting all model parameters at once, a step-wise experimentation procedure was used. Finally two cultivations showed the accuracy of the model.
为了改进百日咳博德特氏菌的培养，并利用监测和控制方面的最新技术，有必要对底物利用进行定量描述。该生物体的生长受到两种主要底物的限制。然而，交互式建模和非交互式建模似乎都不合适。基于实验观察，开发了一种结合基本动力学和增强动力学的模型。不是一次性拟合所有模型参数，而是采用逐步实验程序。最后，两次培养显示了该模型的准确性。
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