在手性固定相上通过填充柱超临界流体色谱法分离布洛芬对映体。

Separation of enantiomers of ibuprofen on chiral stationary phases by packed column supercritical fluid chromatography.

作者信息

Johannsen M

机构信息

Technische Universität Hamburg-Harburg, Arbeitsbereich Verfahrenstechnik II, Germany.

出版信息

J Chromatogr A. 2001 Dec 7;937(1-2):135-8. doi: 10.1016/s0021-9673(01)01330-9.

DOI:10.1016/s0021-9673(01)01330-9

PMID:11765080

Abstract

A packed column supercritical fluid chromatography (SFC) method for the separation of ibuprofen enantiomers on a chiral stationary phase and CO2 with modifier as mobile phase has been developed at an analytical scale. Among 11 different stationary phases the Kromasil CHI-TBB phase showed by far the best separation properties. The influence of different modifiers, injection solvents, temperature, and pressure, and density of the fluid, respectively, on the separation behavior has been studied. It was found that the separation behavior strongly depends on the type of modifier and the modifier content. Temperature and pressure are of less influence.

摘要

已开发出一种填充柱超临界流体色谱（SFC）方法，用于在分析规模下在手性固定相上分离布洛芬对映体，以二氧化碳与改性剂作为流动相。在11种不同的固定相中，Kromasil CHI-TBB相表现出迄今为止最佳的分离性能。分别研究了不同改性剂、进样溶剂、温度、压力以及流体密度对分离行为的影响。结果发现，分离行为强烈依赖于改性剂的类型和改性剂含量。温度和压力的影响较小。