Sing V O, Bartnicki-Garcia S
J Cell Sci. 1975 Oct;19(1):11-20. doi: 10.1242/jcs.19.1.11.
The binding of concanavalin A (Con A) to the cell surface of zoospores and cysts of Phytophthora palmivora was studied by radiometry (125I-Con A), ultraviolet microscopy (fluorescein-Con A) and electron microscopy peroxidase-diaminobenzidine technique). Zoospores were found to secrete during the early stages of encystment a Con A-binding material susceptible to trypsin digestion. This glycoprotein is contained in the so-called peripheral vesicles and is probably responsible for the adhesion of the encysting zoospores to solid surfaces.
利用放射测定法（125I-伴刀豆球蛋白A）、紫外显微镜术（荧光素-伴刀豆球蛋白A）和电子显微镜过氧化物酶-二氨基联苯胺技术，研究了伴刀豆球蛋白A（Con A）与棕榈疫霉游动孢子和孢囊细胞表面的结合情况。研究发现，游动孢子在包囊化早期会分泌一种易被胰蛋白酶消化的Con A结合物质。这种糖蛋白存在于所谓的外周小泡中，可能是包囊化游动孢子附着于固体表面的原因。
