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金色链霉菌孢子形成特异性σ因子σ(rpoZ)指导一个基因的表达,该基因编码的蛋白质类似于参与木质素相关联苯化合物降解的水解酶。

Streptomyces aureofaciens sporulation-specific sigma factor sigma(rpoZ) directs expression of a gene encoding protein similar to hydrolases involved in degradation of the lignin-related biphenyl compounds.

作者信息

Kormanec J, Novakova R, Homerova D, Rezuchova B

机构信息

Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovak Republic.

出版信息

Res Microbiol. 2001 Dec;152(10):883-8. doi: 10.1016/s0923-2508(01)01271-2.

Abstract

A previously established method for the identification of promoters recognized by a heterologous RNA polymerase holoenzyme containing a particular sigma factor was used to identify promoters dependent upon a sporulation specific sigma factor, sigma(RpoZ), of Streptomyces aureofaciens. Three new positive DNA fragments were identified, and these putative rpoZ-dependent promoters, P(ren24), P(ren57), and P(ren71), contained sequences similar to the consensus sequence of flagellar and chemotaxis promoters. However, only P(ren71) was active in S. aureofaciens. The promoter was induced at the time of aerial mycelium formation, and was inactive in an S. aureofaciens strain with an rpoZ-disrupted gene. The results suggest that the P(ren71) promoter is recognized by an RNA polymerase holoenzyme containing sigma(RpoZ) in S. aureofaciens. Sequence analysis of the region directed by P(ren71) revealed a gene, ren71, encoding a protein of 358 amino acids with an Mr 37,770. The deduced protein product showed end-to-end sequence similarity to the meta-cleavage compound hydrolase of Sphingomonas paucimobilis.

摘要

一种先前建立的用于鉴定由含有特定σ因子的异源RNA聚合酶全酶识别的启动子的方法,被用于鉴定依赖于金色链霉菌孢子形成特异性σ因子σ(RpoZ)的启动子。鉴定出了三个新的阳性DNA片段,这些推定的依赖rpoZ的启动子P(ren24)、P(ren57)和P(ren71),含有与鞭毛和趋化性启动子的共有序列相似的序列。然而,只有P(ren71)在金色链霉菌中具有活性。该启动子在气生菌丝形成时被诱导,并且在rpoZ基因被破坏的金色链霉菌菌株中无活性。结果表明,P(ren71)启动子在金色链霉菌中被含有σ(RpoZ)的RNA聚合酶全酶识别。对P(ren71)指导的区域进行序列分析,发现了一个基因ren71,其编码一个由358个氨基酸组成、Mr为37770的蛋白质。推导的蛋白质产物与少动鞘氨醇单胞菌的间位裂解化合物水解酶具有端对端的序列相似性。

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