Crawshaw L I, Wallace H L, Christensen R, Crabbe J C
Department of Biology, Portland State University, Portland, 97207, USA.
Physiol Genomics. 2001 Dec 21;7(2):159-69. doi: 10.1152/physiolgenomics.00041.2001.
The genetic basis for the effects of ethanol on thermoregulation was investigated by utilizing recombinant inbred mouse strains from C57BL/6J and DBA/2J progenitor strains. Changes in core body temperature (T(c)) and the degree of fluctuation of T(c) were monitored in male mice following the administration of ethanol in an environment with cyclic changes in ambient temperature (T(a)). Changes in T(c) were utilized to assess ethanol-induced effects on regulated T(c), whereas fluctuations in T(c) were utilized to assess thermoregulatory disruption. Ethanol was administered intraperitoneally at 1.5, 2.5, and 3.5 g/kg for all strains. Change in T(c) and increase in tail temperature were also evaluated at 2.5 g/kg ethanol in a constant T(a) of 26 degrees C. Associations between the measured physiological responses and previously mapped genetic markers were used to identify quantitative trait loci (QTLs). This established probable chromosome locations for a number of genes for the responses. To our knowledge, this is the first report of QTLs that underlie changes in regulation as well as the disruption of a physiological regulatory system.
通过利用来自C57BL/6J和DBA/2J祖系的重组近交小鼠品系，研究了乙醇对体温调节影响的遗传基础。在环境温度（Ta）呈周期性变化的环境中，对雄性小鼠腹腔注射乙醇后，监测其核心体温（Tc）的变化以及Tc的波动程度。利用Tc的变化来评估乙醇对调节性Tc的诱导作用，而利用Tc的波动来评估体温调节紊乱。对所有品系的小鼠腹腔注射1.5、2.5和3.5 g/kg的乙醇。在26℃的恒定Ta条件下，还评估了2.5 g/kg乙醇剂量下Tc的变化和尾温的升高。通过测量的生理反应与先前定位的遗传标记之间的关联来鉴定数量性状基因座（QTL）。这确定了许多与这些反应相关的基因在染色体上的可能位置。据我们所知，这是关于调节变化以及生理调节系统破坏的QTL的首次报道。
