[快速进展性牙周炎患者中性粒细胞对FMLP和IL-8的趋化反应]

[Chemotactic response of polymorphonuclear neutrophil to FMLP and IL-8 in rapidly progressive periodontitis patients].

作者信息

Liu R, Cao C

机构信息

School of Stomatology, Beijing Medical University, Beijing 100081.

出版信息

Zhonghua Kou Qiang Yi Xue Za Zhi. 1998 Nov;33(6):335-7.

PMID:11774434

Abstract

OBJECTIVE

It has been reported that 48%-85% of rapidly progressive periodontitis (RPP) are associated with a defect in polymorphonuclear neutrophil (PMN) chemotactic function. However, some reports failed to detect any defect in PMN chemotaxis in RPP patients. Further, the influence of race on neutrophil function has been suggested. The purpose of the present study was to determine the PMN chemotactic function in Chinese RPP patients.

METHODS

Neutrophils were obtained from heparinized peripheral blood of 16 RPP and 14 periodontally healthy control subjects matched for age and sex. Cells were isolated by Percoll discontinuous density gradient centrifugation. Chemotaxis was evaluated using modified Boyden chamber. (N-formyl-methionyl-leucyl-phenylalanine, FMLP) (10(-8) mol/L) and IL-8 (100 micrograms/L) which is a more specific neutrophil chemokines were used in this study. The results were determined by staining the filters followed by visual counting under microscopy at 600 x. Data was analyzed using the Students' t test and the value of RPP was compared with that of control subjects(> mean +/- 2s or < mean +/- 2s).

RESULTS AND CONCLUSION

The results showed all RPP patients had normal PMN chemotactic behavior.

摘要

目的

据报道，48%-85%的快速进展性牙周炎（RPP）与多形核中性粒细胞（PMN）趋化功能缺陷有关。然而，一些报告未能检测到RPP患者PMN趋化性存在任何缺陷。此外，有研究表明种族对中性粒细胞功能有影响。本研究的目的是确定中国RPP患者的PMN趋化功能。

方法

从16例RPP患者和14例年龄及性别匹配的牙周健康对照者的肝素化外周血中获取中性粒细胞。通过Percoll不连续密度梯度离心分离细胞。使用改良的Boyden小室评估趋化性。本研究使用了（N-甲酰甲硫氨酰亮氨酰苯丙氨酸，FMLP）（10^(-8)mol/L）和白细胞介素-8（IL-8）（100μg/L），后者是一种更具特异性的中性粒细胞趋化因子。通过对滤膜染色，然后在600倍显微镜下目视计数来确定结果。使用学生t检验分析数据，并将RPP患者的值与对照者的值进行比较（>平均值±2s或<平均值±2s）。