[口腔鳞状细胞癌及癌前病变中端粒酶活性的研究与检测]
[Study and detection of telomerase activity in oral squamous cell carcinomas and precancerous lesions].
作者信息
Yao H, Wu B, Wu Q
机构信息
Department of Stomatology, First Affiliated Hospital of Zhejiang Medical University, Hangzhou 310003.
出版信息
Zhonghua Kou Qiang Yi Xue Za Zhi. 1999 Nov;34(6):328-30.
OBJECTIVE
To find out the expression of telomerase activity in oral squamous cell carcinomas (SCC) and oral precancerous lesions.
METHODS
Telomerase activity was examined by telomerase PCR ELISA assay and telomere repeat amplification protocol (TRAP) electrophoresis. 60 fresh oral specimens were selected, including 20 oral SCC, 30 oral precancerous lesions (10 oral leukoplakia, LK, 20 oral lichen planus, LP) and 10 normal mucosa (NM).
RESULTS
Telomerase activity detected in SCC (0.128) was highest among all of the tissues(LK: 0.054, LP: 0.0094, NM: 0.0014). Statistical difference was notable between SCC and LK, LP, NM (P < 0.01). A little difference was detected between LK, LP and NM. No difference was found between LK, and LP (P > 0.05).
CONCLUSION
Telomerase may play an important role during oncogenesis of oral precancerous lesions, and may be a good molecular biomarker for observing the cancerization ability of precancerous lesion and estimating its prognosis.
目的
探讨端粒酶活性在口腔鳞状细胞癌（SCC）及口腔癌前病变中的表达情况。
方法
采用端粒酶PCR-ELISA法及端粒重复序列扩增法（TRAP）电泳检测端粒酶活性。选取60例新鲜口腔标本，其中包括20例口腔SCC、30例口腔癌前病变（10例口腔白斑，LK，20例口腔扁平苔藓，LP）及10例正常黏膜（NM）。
结果
SCC中端粒酶活性检测值（0.128）在所有组织中最高（LK：0.054，LP：0.0094，NM：0.0014）。SCC与LK、LP、NM之间差异有统计学意义（P < 0.01）。LK、LP与NM之间有细微差异。LK与LP之间无差异（P > 0.05）。
结论
端粒酶可能在口腔癌前病变的发生过程中起重要作用，可能是观察癌前病变癌变能力及评估其预后的良好分子生物标志物。
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