用于从人血浆中去除β2-微球蛋白的免疫吸附剂。

Immunosorbent for removal of b2-microglobulin from human blood plasma.

作者信息

Shabunina I V, Afanas'eva O I, Pokrovskii S N

机构信息

Institute of Experimental Cardiology, Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow.

出版信息

Bull Exp Biol Med. 2001 Oct;132(4):984-6. doi: 10.1023/a:1013679630607.

DOI:10.1023/a:1013679630607

PMID:11782800

Abstract

Immunosorbent removing beta2-microglobulin from human blood plasma was synthesized on the basis of sheep monospecific polyclonal antibodies to beta2-microglobulin and conditions ensuring effective regeneration of the immunosorbent for its repeated use were selected. Relationships between adsorption capacity and the volume of immobilized ligand, antigen concentration in the solution, and duration of incubation were studied. The adsorbent can be used for effective and specific removal of beta2-microglobulin from the plasma of patients on chronic hemodialysis.

摘要

基于针对β2-微球蛋白的羊单特异性多克隆抗体制备了从人血浆中去除β2-微球蛋白的免疫吸附剂，并选择了确保免疫吸附剂有效再生以重复使用的条件。研究了吸附容量与固定化配体体积、溶液中抗原浓度以及孵育时间之间的关系。该吸附剂可用于从慢性血液透析患者的血浆中有效且特异性地去除β2-微球蛋白。