Zhu F, Xing G, He F
Beijing Institute of Radiation Medicine, Beijing 100850, China.
Zhonghua Zhong Liu Za Zhi. 2001 Mar;23(2):115-7.
To explore the suppressive effects of urokinase amino-terminal fragment (ATF) gene on metastatic potential of human breast cancer cell line MCF-7.
A pcDNA3-ATF plasmid containing ATF cDNA under CMV promotor/enhancer control was constructed and transfected into MCF-7 cells by lipofectin. The expression of of uPA/uPAR and ATF in MCF-7 cells were analyzed by RT-PCR and Western blot. The effect of ATF expression on invasiveness in vitro, tumorigenesis and metastasis in vivo of MCF-7 cell was investigated.
MCF-7 cells displayed an overexpression of uPA/uPAR. Expression of ATF was detected after ATF gene-transfection. The invasive capacity of ATF gene-transfected MCF-7 cells was decreased significantly. Although the tumorigenesis was not affected, the in vivo metastasis of ATF gene-transfected MCF-7 cells was remarkably inhibited.
Suppression of invasiveness and metastasis of ATF-transfected MCF-7 cells is perhaps due to a competitive inhibition of interaction with endogenous uPA/uPAR.
探讨尿激酶氨基末端片段（ATF）基因对人乳腺癌细胞系MCF-7转移潜能的抑制作用。
构建在巨细胞病毒启动子/增强子控制下含有ATF cDNA的pcDNA3-ATF质粒，并用脂质体转染MCF-7细胞。采用逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法分析MCF-7细胞中尿激酶型纤溶酶原激活剂（uPA）/尿激酶型纤溶酶原激活剂受体（uPAR）及ATF的表达情况。研究ATF表达对MCF-7细胞体外侵袭能力、体内致瘤性和转移的影响。
MCF-7细胞呈现uPA/uPAR过表达。ATF基因转染后检测到ATF表达。ATF基因转染的MCF-7细胞侵袭能力显著降低。虽然致瘤性未受影响，但ATF基因转染的MCF-7细胞的体内转移受到明显抑制。
ATF转染的MCF-7细胞侵袭和转移受到抑制可能是由于对与内源性uPA/uPAR相互作用的竞争性抑制。
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