[两种黑色素瘤抗原在肝细胞癌中的表达]
[Expression of two types of melanoma antigens in hepatocellular carcinoma].
作者信息
Cai S, Leng X, Wang Y
机构信息
Surgical Department, People's Hospital, Beijing University, Beijing 100044, China.
出版信息
Zhonghua Zhong Liu Za Zhi. 2001 May;23(3):205-7.
OBJECTIVE
To investigate the expression of melanoma antigen MAGE-4 and MAGE-10 mRNA in Chinese human hepatocellular carcinoma (HCC).
METHODS
The expression of MAGE-4 and MAGE-10 mRNA in HCC tissues and the adjacent non-HCC liver tissues was studied using RT-PCR in 48 samples of HCC. Ten samples of cirrhosis and 10 samples of normal liver tissues were examined. The PCR products were sequenced.
RESULTS
Of the 48 HCC samples studied, 15 (31.3%) and 14 (29.2%) expressed MAGE-4 and MAGE-10 mRNA respectively. In contrast, none of the HCC adjacent non-tumorous liver tissues were MAGE-4 and MAGE-10 mRNA detectable. Nor did liver tissues from cirrhosis and normal liver samples. The expression of the two genes in HCC showed no correlation with the serum level of AFP and the tumor size.
CONCLUSION
MAGE-4 and MAGE-10 mRNA are specifically expressed in Chinese HCC samples.
目的
研究黑色素瘤抗原MAGE-4和MAGE-10 mRNA在中国肝癌（HCC）中的表达情况。
方法
采用逆转录聚合酶链反应（RT-PCR）检测48例肝癌组织及其癌旁非肝癌肝组织中MAGE-4和MAGE-10 mRNA的表达，并检测10例肝硬化组织和10例正常肝组织。对PCR产物进行测序。
结果
在48例研究的肝癌样本中，分别有15例（31.3%）和14例（29.2%）表达MAGE-4和MAGE-10 mRNA。相比之下，癌旁非肿瘤肝组织中均未检测到MAGE-4和MAGE-10 mRNA。肝硬化组织和正常肝组织样本中也未检测到。这两个基因在肝癌中的表达与血清甲胎蛋白水平和肿瘤大小无关。
结论
MAGE-4和MAGE-10 mRNA在中国肝癌样本中特异性表达。
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