冷冻和接触冷冻保护剂对牛甲状腺和肾上腺皮质细胞膜中腺苷酸环化酶活性的影响。
The effects of freezing and cryoprotectant exposure on adenylate cyclase activity in cell membranes of bovine thyroid gland and adrenal cortex.
作者信息
Karpenko L G, Gubina N F, Schirova V A, Kaprelyants A S
机构信息
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine, 61015, 23, Pereyaslavskaya Str., Kharkov, Ukraine.
出版信息
Cryo Letters. 2001 Jul-Aug;22(4):229-34.
We present data on the effect of freezing with or without cryoprotectant (CPA) dimethyl sulphoxide (Me(2)SO), as well as effects of exposure to CPA alone on the activity of adenylate cyclase in cellular membranes of thyroid glands and adrenal cortex. Our results show that freezing without cryoprotectant increases unstimulated adenylate cyclase activity and decreases sensitivity to specific regulators. CPA at a concentration of 5% for adenylate cyclase in adrenal cortex tissue and of 10% in thyroid gland allowed preservation of the best responses to specific receptor agonists.
我们展示了关于添加或不添加冷冻保护剂（CPA）二甲基亚砜（Me(2)SO）进行冷冻的效果的数据，以及单独暴露于CPA对甲状腺和肾上腺皮质细胞膜中腺苷酸环化酶活性的影响。我们的结果表明，不使用冷冻保护剂进行冷冻会增加未受刺激的腺苷酸环化酶活性，并降低对特定调节因子的敏感性。对于肾上腺皮质组织中的腺苷酸环化酶，5%浓度的CPA以及对于甲状腺中的腺苷酸环化酶，10%浓度的CPA能够使对特定受体激动剂的最佳反应得以保留。
Zotero 插件