围产期暴露于多氯联苯混合物1254的大鼠的闪光诱发电位、体感诱发电位和外周神经诱发电位

Flash-, somatosensory-, and peripheral nerve-evoked potentials in rats perinatally exposed to Aroclor 1254.

作者信息

Herr D W, Graff J E, Derr-Yellin E C, Crofton K M, Kodavanti P R

机构信息

Neurotoxicology Division, MD-74B, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711, USA.

出版信息

Neurotoxicol Teratol. 2001 Nov-Dec;23(6):591-601. doi: 10.1016/s0892-0362(01)00180-5.

DOI:10.1016/s0892-0362(01)00180-5

PMID:11792527

Abstract

Pregnant Long-Evans rats were exposed to 0, 1 or 6 mg/kg/day of Aroclor 1254 (A1254; Lot no. 124-191), a commercial mixture of polychlorinated biphenyls (PCBs), from gestation day (GD) 6 through postnatal day (PND) 21. At 128-140 days of age, male and female offspring were tested for visual-, somatosensory- and peripheral nerve-evoked potentials. The evoked responses increased in amplitude with larger stimulus intensities, and gender differences were detected for some endpoints. In contrast, developmental exposure to A1254 failed to significantly affect the electrophysiological measures. A subset of the animals were tested for low-frequency hearing dysfunction using reflex modification audiometry (RMA). An elevated threshold for a 1-kHz tone was observed, replicating previous findings of A1254-induced auditory deficits [Hear. Res. 144 (2000) 196; Toxicol. Sci. 45(1) (1998) 94; Toxicol. Appl. Pharmacol. 135(1) (1995) 77.]. These findings indicate no statistically significant changes in visual-, somatosensory- or peripheral nerve-evoked potentials following developmental exposure to doses of A1254 that produce behavioral hearing deficits. However, subtle changes in the function of the visual or somatosensory systems cannot be disproved.

摘要

将怀孕的Long-Evans大鼠从妊娠第6天（GD）至出生后第21天（PND）暴露于0、1或6 mg/kg/天的多氯联苯（PCBs）商业混合物Aroclor 1254（A1254；批号124-191）中。在128-140日龄时，对雄性和雌性后代进行视觉、体感和外周神经诱发电位测试。诱发电反应的幅度随刺激强度增大而增加，并且在一些终点检测到了性别差异。相比之下，发育过程中暴露于A1254并未显著影响电生理指标。使用反射修正听力测定法（RMA）对一部分动物进行低频听力功能障碍测试。观察到1 kHz音调的阈值升高，重复了先前关于A1254诱导听觉缺陷的研究结果[《听觉研究》144（2000）196；《毒理学科学》45(1)（1998）94；《毒理学与应用药理学》135(1)（1995）77]。这些研究结果表明，发育过程中暴露于产生行为性听力缺陷剂量的A1254后，视觉、体感或外周神经诱发电位无统计学显著变化。然而，视觉或体感系统功能的细微变化不能被排除。