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运动可诱导糖尿病大鼠中CLUT4基因表达及蛋白质含量增加。

[Exercise induces increased CLUT4 gene expression and protein content in diabetic rats].

作者信息

Wu Y, Yang X, Li Y

机构信息

Department of Sports Medicine, Huashan Hospital, Shanghai Medical University, Shanghai 200040 China.

出版信息

Zhonghua Yi Xue Za Zhi. 2000 Mar;80(3):172-4.

PMID:11798751

Abstract

OBJECTIVE

To investigate the effects of exercise on insulin receptor, glucose transporter 4 (GLUT4) gene expression and protein content in skeletal muscle cells of streptozotocin-induced diabetic rats.

METHODS

All rats were randomized into three groups: normal, diabetic and diabetic exercise groups. Diabetic exercise rats were swim-trained for 6 weeks. The GLUT4 mRNA of skeletal muscle was determined with dot blot and GLUT4 protein was detected by Western blotting.

RESULTS

After 6 weeks of exercise training, plasma glucose level of diabetic rats decreased from 18.5 mmol/L +/- 1.9 mmol/L to 14.0 mmol/L +/- 3.3 mmol/L (P < 0.01). The dot blot revealed that GLUT4 mRNA decreased by 54.9% in skeletal muscle cells of diabetic rats compared with the normal rats. The Western blotting showed that GLUT4 protein of skeletal muscle cell in diabetic rats decreased by 30.7% compared with the control rats. GLUT4 mRNA and the protein of skeletal muscle in diabetic exercise group increased by 56% and 60.8% respectively as compared with the diabetic rats. However, the maximum binding of insulin receptor of skeletal muscle cells in diabetic exercise group decreased significantly compared with diabetic group.

CONCLUSION

The weaker expression of GLUT4 gene and the lower level of GLUT4 protein may be one of the causes of hyperglycemia in diabetic rats. Exercise training may raise the GLUT4 gene expression and the amount of GLUT4 protein in diabetic rats, contributing to the main mechanism of improved post-receptor insulin resistance and decreased hyperglycemia in diabetic rats.

摘要

目的

探讨运动对链脲佐菌素诱导的糖尿病大鼠骨骼肌细胞胰岛素受体、葡萄糖转运蛋白4（GLUT4）基因表达及蛋白含量的影响。

方法

将所有大鼠随机分为三组：正常组、糖尿病组和糖尿病运动组。糖尿病运动组大鼠进行为期6周的游泳训练。采用斑点杂交法测定骨骼肌中GLUT4 mRNA水平，用蛋白质免疫印迹法检测GLUT4蛋白含量。

结果

运动训练6周后，糖尿病大鼠的血糖水平从18.5 mmol/L±1.9 mmol/L降至14.0 mmol/L±3.3 mmol/L（P<0.01）。斑点杂交显示，糖尿病大鼠骨骼肌细胞中GLUT4 mRNA较正常大鼠降低了54.9%。蛋白质免疫印迹结果表明，糖尿病大鼠骨骼肌细胞中GLUT4蛋白较对照组降低了30.7%。与糖尿病组相比，糖尿病运动组大鼠骨骼肌中GLUT4 mRNA和蛋白含量分别增加了56%和60.8%。然而，糖尿病运动组大鼠骨骼肌细胞胰岛素受体最大结合力较糖尿病组显著降低。