Celik C, Amanvermez R, Ozkan K
Department of Biochemistry, Ondokuz Mayis University, 55139 Kurupelit/Samsun, Turkey.
Parasite. 2001 Dec;8(4):343-8. doi: 10.1051/parasite/2001084343.
The aim of this study was to search and compare free amino acid composition of fertile and infertile cyst fluids obtained from humans and animals infected naturally with Echinococcus granulosus, by using automated analysis based on cation-exchange chromatography with post-column ninhydrin derivatization system. 11 free amino acids from fertile (sheep origin), nine from infertile (cattle origin), 13 from infertile (human origin) hydatid cyst fluids and 19 amino acids from sera of patients with hydatid infection were detected. The levels of glycine, alanine, valine and lyrosine in fertile and infertile hydatid cysts fluids were significantly higher than in sera from patients with hydatid cysts. Glycine level in the fertile hydatid cyst fluids (sheep origin) was significantly higher than those of infertile cysts fluids (cattle and human origin) and sera with hydatid patients. Glycine level in fertile hydatid cyst fluids was about two times more concentrated in infertile cattle cyst fluids, 10 times more concentrated in infertile human hydatid cyst fluids and 13 times more concentrated in sera with hydatid patients. On the other hand, alanine and valine concentration in the fertile and infertile cyst fluids were at similar level with the exception that valine level in fertile cyst fluids was 12 times more concentrated in infertile human cyst fluids. The levels of tyrosine, citrulline, leucine, isoleucine and lysine amino acids in fertile and infertile hydatid cyst fluids were similar. Our findings with respect to fertile and infertile cysts fluids showed that free amino acids concentrations in cyst fluids were significantly, higher in sera from patients with hydatid cyst. Total amount of free amino acids content in fertile and infertile cyst fluids was three to eight times higher from that of human sera with hydatid patients.
本研究的目的是通过基于阳离子交换色谱和柱后茚三酮衍生化系统的自动分析，搜索并比较从自然感染细粒棘球绦虫的人类和动物中获取的有生育力和无生育力的包虫囊肿液中的游离氨基酸组成。检测到来自有生育力（绵羊来源）的包虫囊肿液中有11种游离氨基酸，来自无生育力（牛来源）的有9种，来自无生育力（人类来源）的包虫囊肿液中有13种，以及来自包虫感染患者血清中的19种氨基酸。有生育力和无生育力的包虫囊肿液中甘氨酸、丙氨酸、缬氨酸和酪氨酸的水平显著高于包虫囊肿患者的血清。有生育力的包虫囊肿液（绵羊来源）中的甘氨酸水平显著高于无生育力的囊肿液（牛和人类来源）以及包虫患者的血清。有生育力的包虫囊肿液中的甘氨酸水平在无生育力的牛囊肿液中浓缩约两倍，在无生育力的人类包虫囊肿液中浓缩10倍，在包虫患者血清中浓缩13倍。另一方面，有生育力和无生育力的囊肿液中丙氨酸和缬氨酸的浓度处于相似水平，只是有生育力的囊肿液中的缬氨酸水平在无生育力的人类囊肿液中浓缩12倍。有生育力和无生育力的包虫囊肿液中酪氨酸、瓜氨酸、亮氨酸、异亮氨酸和赖氨酸的水平相似。我们关于有生育力和无生育力的囊肿液的研究结果表明，囊肿液中的游离氨基酸浓度显著高于包虫囊肿患者的血清。有生育力和无生育力的囊肿液中游离氨基酸含量的总量比有包虫病的人类血清高3至8倍。
