Liu J, Chen P
Department of Respiratory Medicine, The Second Affiliated Hospital of Central South University, Changsha 410011, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2001 May;24(5):280-2.
To assess the relationship between the expressions of lymphocyte function associated antigen-1 (LFA-1) in macrophages in both BALF and bronchial mucosa and the severity of airway inflammation in patients of chronic bronchitis.
Bronchial alveolar lavage and bronchial mucosa biopsy were performed in 18 patients with stable stage of chronic bronchitis (CB). LFA-1 positive alveolar macrophage (AM) was measured by immunocytochemistry stain. The percentages and number of AM in BALF and bronchial mucosa were also calculated and the thickness of mucosa measured.
(1) The numbers of AM and neutrophils of BALF in CB were significantly higher than those in healthy group (P < 0.01, respectively). So did the percentage of neutrophils of BALF (all P < 0.05). (2) The number [(4.36 +/- 2.11) x 10(4)/ml] and percentage [(24.6 +/- 5.8)%] of positive AM expressing LFA-1 in CB were significantly higher than those [(0.63 +/- 0.25) x 10(4)/ml, (10.6 +/- 2.7)%] in healthy group (all P < 0.01, respectively). The number of AM in BALF was positively correlated with that of positive AM expressing LFA-1 (r = 0.624 7, P < 0.01). The number of CD(68)(+) cells [(21.6 +/- 4.6)/HP] in mucosa and the thickness of mucosa [(0.20 +/- 0.09) micrometer] in CB were significantly greater than those [(10.0 +/- 3.4)/HP, (0.08 +/- 0.04) micrometer] in healthy group (P < 0.01, respectively). They were inversely corrlated with the value of FEV(1)%pre (r = -0.5875, P < 0.01; r = -0.655, P < 0.05).
Increased expression of LFA-1 may be one of important causes leading to the accumulation and migration of AM in the airway of patient with CB. AM infiltration and the thickening of bronchial mucosa are closely related to the decline of FEV(1).
评估慢性支气管炎患者支气管肺泡灌洗液（BALF）及支气管黏膜中巨噬细胞淋巴细胞功能相关抗原-1（LFA-1）的表达与气道炎症严重程度之间的关系。
对18例慢性支气管炎（CB）稳定期患者进行支气管肺泡灌洗和支气管黏膜活检。采用免疫细胞化学染色法检测LFA-1阳性肺泡巨噬细胞（AM）。计算BALF和支气管黏膜中AM的百分比及数量，并测量黏膜厚度。
（1）CB患者BALF中AM和中性粒细胞数量均显著高于健康组（分别为P < 0.01）。BALF中中性粒细胞百分比亦如此（均P < 0.05）。（2）CB患者中表达LFA-1的阳性AM数量[（4.36±2.11）×10⁴/ml]及百分比[（24.6±5.8）%]均显著高于健康组[（0.63±0.25）×10⁴/ml，（10.6±2.7）%]（均P < 0.01）。BALF中AM数量与表达LFA-1的阳性AM数量呈正相关（r = 0.624 7，P < 0.01）。CB患者黏膜中CD68⁺细胞数量[（21.6±4.6）/HP]及黏膜厚度[（0.20±0.09）μm]均显著大于健康组[（10.0±3.4）/HP，（0.08±0.04）μm]（分别为P < 0.01）。它们与FEV₁%预计值呈负相关（r = -0.5875，P < 0.01；r = -0.655，P < 0.05）。
LFA-1表达增加可能是导致CB患者气道中AM聚集和迁移的重要原因之一。AM浸润和支气管黏膜增厚与FEV₁下降密切相关。
