用亲和色谱法纯化猪肠激酶。
Purification of porcine enterokinase by affinity chromatography.
作者信息
Grant D A, Hermon-Taylor J
出版信息
Biochem J. 1975 May;147(2):363-6. doi: 10.1042/bj1470363.
Abstract
A method is described for the purification of porcine enterokinase by affinity chromatography with p-aminobenzamidine as the ligand. Purification was completed by immunoadsorption with antisera raised to components binding non-biologically to the gel. The final enterokinase preparation was 2.3 times more active than the most active preparation previously described.
摘要
描述了一种用对氨基苯甲脒作为配体通过亲和色谱法纯化猪肠激酶的方法。通过用针对与凝胶非生物结合的组分产生的抗血清进行免疫吸附来完成纯化。最终的肠激酶制剂的活性比先前描述的最具活性的制剂高2.3倍。
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