Stege H
Clinical and Experimental Photodermatology, Department of Dermatology, University of Düsseldorf, Moorenstrasse 5, 40225 Düsseldorf, Germany.
J Photochem Photobiol B. 2001 Dec 31;65(2-3):105-8. doi: 10.1016/s1011-1344(01)00246-9.
Exposure to ultraviolet B (UVB) radiation leads to an increased generation of UVB-induced skin damage in humans. The most important UVB-induced side effects are UVB-induced immunosuppression and photocarcinogenesis and there is a large body of evidence that cyclobutane pyrimidine dimers (CPD) induced by UVB radiation play a pivotal role in both processes. The topical application of DNA repair enzymes is a new innovative strategy to reduce the amount of CPDs in human skin. Two different methods have recently been established. The use of T4 endonuclease V was of clinical efficacy in protecting patients with a nucleotide excision repair defect from premalignant and malignant skin lesions. Application of photolyase, a xenogenic enzyme which has been found in different organisms is also capable of removing UVB-induced CPD from normal human skin cells in vivo and appears to be more effective than T4 endonuclease V in damage removal. Photolyase encapsulated in liposomes may have in the near future a broad use as an active ingredient in modern skin care products.
暴露于紫外线B（UVB）辐射会导致人类皮肤中UVB诱导的损伤增加。最重要的UVB诱导的副作用是UVB诱导的免疫抑制和光致癌作用，并且有大量证据表明UVB辐射诱导的环丁烷嘧啶二聚体（CPD）在这两个过程中都起着关键作用。局部应用DNA修复酶是一种减少人体皮肤中CPD数量的新型创新策略。最近已经建立了两种不同的方法。使用T4内切核酸酶V在保护患有核苷酸切除修复缺陷的患者免受癌前和恶性皮肤病变方面具有临床疗效。光解酶是一种在不同生物体中发现的异种酶，它也能够在体内从正常人皮肤细胞中去除UVB诱导的CPD，并且在损伤去除方面似乎比T4内切核酸酶V更有效。包裹在脂质体中的光解酶在不久的将来可能会作为现代护肤品中的活性成分得到广泛应用。
