在正常恒河猴的淋巴组织中，解剖部位和免疫功能与相关细胞因子mRNA表达水平相关。

Anatomic site and immune function correlate with relative cytokine mRNA expression levels in lymphoid tissues of normal rhesus macaques.

作者信息

Abel K, Alegria-Hartman M J, Zanotto K, McChesney M B, Marthas M L, Miller C J

机构信息

Center for Comparative Medicine, University of California Davis, Davis, CA 95616, USA.

出版信息

Cytokine. 2001 Dec 7;16(5):191-204. doi: 10.1006/cyto.2001.0961.

DOI:10.1006/cyto.2001.0961

PMID:11814315

Abstract

Reverse transcriptase real-time polymerase chain reaction was used to determine pro-inflammatory, anti-viral and immunoregulatory cytokine mRNA expression levels in peripheral blood mononuclear cells (PBMC) of healthy juvenile, adolescent and adult rhesus macaques. Few age-related changes in cytokine mRNA expression levels were observed. Expression of interleukin 2 and Mx, a type I interferon-inducible gene, decreased with age, whereas interleukin 4 and macrophage inflammatory protein 1 (MIP-1) alpha and beta mRNA levels increased in older monkeys. Independent of age, the pro-inflammatory cytokines [tumour necrosis factor alpha (TNF-alpha) and chemokines] were expressed at higher mRNA levels in PBMC than the immunoregulatory cytokines (interleukins 2, 4, 12). Pro-inflammatory cytokine mRNA expression levels were highest in lymphoid tissues draining mucosal surfaces. Thus, a correlation exists between cytokine mRNA levels in lymphoid tissues and the anatomical site.

摘要

采用逆转录实时聚合酶链反应来测定健康幼年、青少年和成年恒河猴外周血单个核细胞（PBMC）中促炎、抗病毒和免疫调节细胞因子的mRNA表达水平。观察到细胞因子mRNA表达水平几乎没有与年龄相关的变化。白细胞介素2和Mx（一种I型干扰素诱导基因）的表达随年龄下降，而老年猴子中白细胞介素4和巨噬细胞炎性蛋白1（MIP-1）α和β的mRNA水平升高。与年龄无关，促炎细胞因子[肿瘤坏死因子α（TNF-α）和趋化因子]在PBMC中的mRNA表达水平高于免疫调节细胞因子（白细胞介素2、4、12）。促炎细胞因子mRNA表达水平在引流黏膜表面的淋巴组织中最高。因此，淋巴组织中的细胞因子mRNA水平与解剖部位之间存在相关性。