Pavlícek Adam, Paces Jan, Clay Oliver, Bernardi Giorgio
Laboratoire de Génétique Moléculaire, Institut Jacques Monod, 75005 Paris, France.
FEBS Lett. 2002 Jan 30;511(1-3):165-9. doi: 10.1016/s0014-5793(01)03283-5.
Prior to genome sequencing, information on base composition (GC level) and its variation in mammalian genomes could be obtained using density gradient ultracentrifugation. Analyses using this approach led to the conclusion that mammalian genomes are organized into mosaics of fairly homogeneous regions, called isochores. We present an initial compositional overview of the chromosomes of the recently available draft human genome sequence, in the form of color-coded moving window plots and corresponding GC level histograms. Results obtained from the draft human genome sequence agree well with those obtained or deduced earlier from CsCl experiments. The draft sequence now permits the visualization of the mosaic organization of the human genome at the DNA sequence level.
在进行基因组测序之前，可以使用密度梯度超速离心法获得有关哺乳动物基因组碱基组成（GC水平）及其变异的信息。使用这种方法进行的分析得出结论，哺乳动物基因组被组织成相当均匀的区域镶嵌体，称为等密度区带。我们以颜色编码的移动窗口图和相应的GC水平直方图的形式，展示了最近可用的人类基因组序列草图中各染色体的初步组成概况。从人类基因组序列草图中获得的结果与早期通过CsCl实验获得或推断出的结果非常吻合。现在，该序列草图使我们能够在DNA序列水平上直观地看到人类基因组的镶嵌组织。
