用于核酸的横向弛豫优化的HCN实验：结合TROSY和MQ自旋演化的优势。

Transverse relaxation optimized HCN experiment for nucleic acids: combining the advantages of TROSY and MQ spin evolution.

作者信息

Brutscher B, Simorre J P

机构信息

Institut de Biologie Structurale, Jean-Pierre Ebel CNRS-CEA, Grenoble, France.

出版信息

J Biomol NMR. 2001 Dec;21(4):367-72. doi: 10.1023/a:1013398728535.

DOI:10.1023/a:1013398728535

PMID:11824756

Abstract

A three-dimensional MQ-TROSY-HCN pulse sequence is presented which provides intra-base and sugar-to-base correlations for 13C, 15N labeled nucleic acids (RNA, DNA). The experiment simultaneously exploits the favorable relaxation properties of 1H-13C multiple quantum coherence for sugar carbons and of 13C TROSY-type spin evolution for base carbons. MQ-TROSY-HCN thus combines the advantages of MQ-HCN for sugar-to-base and TROSY-HCN for intra-base correlations in a single experiment. In addition, two slightly different implementations of the MQ-TROSY-HCN experiment ensure optimal performance for small and larger oligonucleotides, respectively. The advantages of the MQ-TROSY-HCN experiment compared to the best previous implementations of HCN are demonstrated for a 33 nucleotide RNA aptamer.

摘要

本文提出了一种三维MQ-TROSY-HCN脉冲序列，该序列可为13C、15N标记的核酸（RNA、DNA）提供碱基内以及糖基与碱基之间的相关性。该实验同时利用了糖环碳原子的1H-13C多量子相干的有利弛豫特性以及碱基碳原子的13C TROSY型自旋演化。因此，MQ-TROSY-HCN在一个实验中结合了MQ-HCN用于糖基与碱基相关性以及TROSY-HCN用于碱基内相关性的优点。此外，MQ-TROSY-HCN实验的两种略有不同的实现方式分别确保了对小寡核苷酸和大寡核苷酸的最佳性能。对于一个33个核苷酸的RNA适配体，展示了MQ-TROSY-HCN实验相对于之前最佳的HCN实现方式的优势。

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用于核酸的横向弛豫优化的HCN实验：结合TROSY和MQ自旋演化的优势。

Transverse relaxation optimized HCN experiment for nucleic acids: combining the advantages of TROSY and MQ spin evolution.

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