Mawassi M, Karasev A V, Mietkiewska E, Gafny R, Lee R F, Dawson W O, Bar-Joseph M
Department of Virology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.
Virology. 1995 Apr 1;208(1):383-7. doi: 10.1006/viro.1995.1165.
Preparations of single-stranded (ss) RNA extracted from particles of the Israeli VT strain of citrus tristeza virus (CTV-VT), and ss- and double-stranded (ds) RNA preparations extracted from infected Alemow (Citrus macrophylla) plants, contained a population of molecules with features that suggest that they are defective RNAs. The prototype of 2424 nt was cloned and sequenced and was found to be composed of two genomic regions corresponding to the 5' (1151 nt) and the 3' (1259 nt) termini of the genomic CTV-RNA, with two perfect direct repeats of eight nucleotides of unknown origin at the junction site. Northern hybridization analysis demonstrated that this 2.4-kb defective RNA is an abundant species among the other CTV-specific ss- and ds-RNAs in infected plants. The 2.4-kb RNA was found encapsidated by the CTV coat protein indicating that the CTV origin of assembly is located close to the 5' or 3' terminus. This is the first defective RNA to be reported for a member of the closterovirus group.
从以色列柑橘衰退病毒VT株（CTV-VT）颗粒中提取的单链（ss）RNA制剂，以及从受感染的阿蕾檬（Citrus macrophylla）植株中提取的ssRNA和双链（ds）RNA制剂，含有一群具有某些特征的分子，这些特征表明它们是缺陷RNA。克隆并测序了2424个核苷酸的原型，发现它由两个基因组区域组成，分别对应于基因组CTV-RNA的5'端（1151个核苷酸）和3'端（1259个核苷酸），在连接位点有两个来自未知来源的8个核苷酸的完美正向重复序列。Northern杂交分析表明，这种2.4kb的缺陷RNA在受感染植株中其他CTV特异性ssRNA和dsRNA中是丰富的种类。发现2.4kb的RNA被CTV外壳蛋白包裹，这表明组装的CTV起源位于靠近5'或3'端的位置。这是首次报道的属于长线形病毒组成员的缺陷RNA。
