[蛋白质表达对血管平滑肌细胞反义c-myc和增殖细胞核抗原的影响]
[Effect of protein expression on antisense c-myc and PCNA for vascular smooth muscle cells].
作者信息
Bian J, Zhang B, Qian H, Chen S
机构信息
Department of Vascular Surgery, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
出版信息
Zhonghua Wai Ke Za Zhi. 2000 Feb;38(2):140-2.
OBJECTIVE
To study the methods of inhibiting proliferation of vascular smooth muscle cells (VSMCs), we observed the change of protein expression after using antisense c-myc, PCNA for VSMCs.
METHODS
The sense, antisense, and mismatched oligomers for c-myc or PCNA were individually delivered to the cultured VSMCs. Using Western blot, immunohistochemistry assay and image analysis system, we observed the change of c-myc and PCNA protein expression in the VSMCs.
RESULTS
Antisense c-myc, PCNA at concentration of 10 micromol/L can successfully inhibit the expression of c-myc, PCNA protein in the VSMCs (P < 0.05), but the sense and the mismatched do not have this effect (P > 0.05).
CONCLUSION
The data suggested that the antisense c-myc, PCNA could attenuate the protein expression of c-myc, PCNA, and it is an effective method for the inhibition of VSMCs hyperplasia.
目的
为研究抑制血管平滑肌细胞（VSMCs）增殖的方法，我们观察了对VSMCs使用反义c-myc、增殖细胞核抗原（PCNA）后蛋白质表达的变化。
方法
将c-myc或PCNA的正义、反义及错配寡核苷酸分别导入培养的VSMCs。采用蛋白质免疫印迹法、免疫组织化学法及图像分析系统，观察VSMCs中c-myc和PCNA蛋白表达的变化。
结果
浓度为10微摩尔/升的反义c-myc、PCNA可成功抑制VSMCs中c-myc、PCNA蛋白的表达（P<0.05），但正义及错配寡核苷酸无此作用（P>0.05）。
结论
数据表明反义c-myc、PCNA可减弱c-myc、PCNA的蛋白表达，是抑制VSMCs增生的有效方法。
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