Luo Q, Wang L, Feng X, Cai R, Shen G, Deng Y, Xia R
Department of Ophthalmology, Affiliated First Hospital, West China University of Medical Sciences, Chengdu 610041.
Zhonghua Yan Ke Za Zhi. 1999 Jul;35(4):268-70, 17.
To investigate the histopathological changes of lattice, granular and macular corneal dystrophy.
Corneal buttons were obtained from penetrating keratoplasty due to severe corneal dystrophy, and their sections and ultrathin sections with several special stains were observed under a light microscope (LM) and electron microscope (EM).
In cases with lattice dystrophy, the stromal fusiform deposits seen under LM were confirmed to be amorphous materiel with filaments by EM and stained positively for Masson trichrome, PAS and Congo red. In cases with granular dystrophy, the granular or bread debris deposits found between laminae under LM were extracellular material observed by EM and they were stained positively for Masson trichrome and alcian blue. In cases with macular dystrophy, the stromal spheroid or macular deposits were seen under LM, and there were numerous vacuoles containing fibrils and granules in the cytoplasm of keratocytes and accumulative amorphous material in the stroma under EM; the deposits were positive for colloidal iron and alcian blue stain.
In corneal lattice dystrophy, there are amyloid deposits; in granular dystrophy, there are extracellular material deposits; and in macular dystrophy, there is mucopolysaccharide accumulation.
研究格子状、颗粒状及斑状角膜营养不良的组织病理学变化。
因严重角膜营养不良行穿透性角膜移植术获取角膜植片，对其切片及经多种特殊染色的超薄切片分别在光学显微镜（LM）和电子显微镜（EM）下进行观察。
在格子状营养不良病例中，光学显微镜下所见的基质梭形沉积物经电子显微镜证实为含细丝的无定形物质，对三色染色、过碘酸雪夫染色（PAS）及刚果红染色呈阳性。在颗粒状营养不良病例中，光学显微镜下在板层间发现的颗粒状或面包屑样沉积物经电子显微镜观察为细胞外物质，对三色染色及阿尔辛蓝染色呈阳性。在斑状营养不良病例中，光学显微镜下可见基质球形或斑状沉积物，电子显微镜下可见角膜细胞胞质内有许多含原纤维和颗粒的空泡以及基质中累积的无定形物质；沉积物对胶态铁和阿尔辛蓝染色呈阳性。
角膜格子状营养不良中有淀粉样沉积物；颗粒状营养不良中有细胞外物质沉积；斑状营养不良中有黏多糖蓄积。
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