Abdel-Wanis M el-S, Tsuchiya H, Kawahara N, Tomita K
Orthopaedic Department, Faculty of Medicine, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa 920-8641, Japan.
J Orthop Sci. 2001;6(5):424-9. doi: 10.1007/s007760170009.
In the field of spinal tumors, intralesional tumor cutting is sometimes inevitable. The purpose of this study was to compare the potential for tumor growth after intralesional cutting by T-saw, Gigli saw, and scalpel. Tumors, prepared by the subcutaneous injection of human HT 1080 fibrosarcoma cells in nude mice, were harvested and cut with a T-saw, Gigli saw, or scalpel. A 3-cm wound was created in the skin on the back in another group of nude mice. The cut surface of the tumor was rubbed for 10 s against the subcutaneous tissue in this second group of nude mice. In the same manner, the instrument used for tumor cutting was rubbed against the subcutaneous tissue in the 3-cm wound in the back of a third group of nude mice for 10 s. Other instruments used for tumor cutting were immediately washed in culture medium, and the total number of tumor cells was counted. Tumor blocks and single-cell suspensions prepared from tumor tissues of the same weight were inoculated and injected into a fourth group of nude mice. The incidence of tumor growth after the rubbing of the subcutaneous tissue with the tumor surface cut with a T-saw, Gigli saw, and scalpel was 16.7%, 50.0%, and 33.3%, respectively. The corresponding figures after the rubbing of the subcutaneous tissue with a T-saw, Gigli saw, and scalpel was 16.7%, 33.3%, and 33.3%, respectively. The mean numbers of tumor cells attached to the T-saw, Gigli saw, and scalpel were 1.88 x 105, 5.02 x 105, and 4.92 x 105, respectively. Finally, the incidence of tumor growth after the inoculation of tumor blocks and single-cell suspensions was 100% and 77.8%, respectively. These findings show that tumor recurrence is less likely after intralesional tumor cutting with a T-saw than after such cutting with a Gigli saw or scalpel.
在脊柱肿瘤领域，瘤内肿瘤切除有时不可避免。本研究的目的是比较用线锯、Gigli锯和手术刀进行瘤内切除后肿瘤生长的可能性。通过在裸鼠皮下注射人HT 1080纤维肉瘤细胞制备肿瘤，然后用线锯、Gigli锯或手术刀进行切割。在另一组裸鼠的背部皮肤上制造一个3厘米的伤口。在第二组裸鼠中，将肿瘤的切割面与皮下组织摩擦10秒。同样地，用于肿瘤切割的器械在第三组裸鼠背部3厘米伤口的皮下组织上摩擦10秒。用于肿瘤切割的其他器械立即在培养基中冲洗，并对肿瘤细胞总数进行计数。将由相同重量的肿瘤组织制备的肿瘤块和单细胞悬液接种并注入第四组裸鼠。用线锯、Gigli锯和手术刀切割肿瘤表面后与皮下组织摩擦，肿瘤生长的发生率分别为16.7%、50.0%和33.3%。用线锯、Gigli锯和手术刀与皮下组织摩擦后的相应数字分别为16.7%、33.3%和33.3%。附着在线锯、Gigli锯和手术刀上的肿瘤细胞平均数分别为1.88×10⁵、5.02×10⁵和4.92×10⁵。最后，接种肿瘤块和单细胞悬液后肿瘤生长的发生率分别为100%和77.8%。这些发现表明，与用Gigli锯或手术刀进行瘤内肿瘤切除相比，用线锯进行瘤内肿瘤切除后肿瘤复发的可能性较小。
