Suzuki Yoshikazu, Tani Tomomi, Sutoh Kazuo, Kamimura Shinji
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba 3-8-1, Meguro, 153-8902, Tokyo, Japan.
FEBS Lett. 2002 Feb 13;512(1-3):235-9. doi: 10.1016/s0014-5793(02)02269-x.
We have devised a novel method to visualize the fluorescence spectrum of a single fluorescent molecule using prism-based spectroscopy. Equipping a total internal reflection microscope with a newly designed wedge prism, we obtained a spectral image of a single rhodamine red molecule attached to an essential light chain of myosin. We also obtained a spectral image of single-pair fluorescence resonance energy transfer between rhodamine red and Cy5 in a double-labeled myosin motor domain. This method could become a useful tool to investigate the dynamic processes of biomolecules at the single-molecule level.
我们设计了一种新颖的方法,利用基于棱镜的光谱技术来可视化单个荧光分子的荧光光谱。通过给全内反射显微镜配备新设计的楔形棱镜,我们获得了附着在肌球蛋白轻链上的单个若丹明红分子的光谱图像。我们还获得了双标记肌球蛋白运动结构域中若丹明红与Cy5之间单对荧光共振能量转移的光谱图像。该方法可能成为在单分子水平上研究生物分子动态过程的有用工具。