幽门螺杆菌鞭毛蛋白A基因的多态性
Polymorphism of flagellin A gene in Helicobacter pylori.
作者信息
Ji W S, Hu J L, Qiu J W, Peng D R, Shi B L, Zhou S J, Wu K C, Fan D M
机构信息
Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
出版信息
World J Gastroenterol. 2001 Dec;7(6):783-7. doi: 10.3748/wjg.v7.i6.783.
AIM
To study the polymorphism of flagellin A genotype and its significance in Helicobacter pylori (H. pylori).
METHODS
As the template, genome DNA was purified from six clinical isolates of H. pylori from outpatients, and the corresponding flagellin A fragments were amplified by polymerase chain reaction. All these products were sequenced. These sequences were compared with each other, and analyzed by software of FASTA program.
RESULTS
Specific PCR products were amplified from all of these H. pylori isolates and no length divergence was found among them. Compared with each other, the highest ungapped identity is 99.10%, while the lowest is 94.65%. Using FASTA program, the alignments between query and library sequences derived from different H. pylori strains were higher than 90%.
CONCLUSION
The nucleotide sequence of flagellin A in H. pylori is highly conservative with incident divergence. This information may be useful for gene diagnosis and further study on flagellar antigen phenotype.
目的
研究幽门螺杆菌鞭毛蛋白A基因型的多态性及其意义。
方法
以门诊患者的6株幽门螺杆菌临床分离株的基因组DNA为模板进行纯化，通过聚合酶链反应扩增相应的鞭毛蛋白A片段。对所有产物进行测序。将这些序列相互比较，并通过FASTA程序软件进行分析。
结果
所有幽门螺杆菌分离株均扩增出特异性PCR产物，且它们之间未发现长度差异。相互比较时，最高无间隙同一性为99.10%，最低为94.65%。使用FASTA程序，来自不同幽门螺杆菌菌株的查询序列与文库序列之间的比对率高于90%。
结论
幽门螺杆菌鞭毛蛋白A的核苷酸序列高度保守，但存在偶然差异。该信息可能有助于基因诊断及对鞭毛抗原表型的进一步研究。
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