Xie Q, Liu K D, Hu M Y, Zhou K
Experimental Research Center of Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
World J Gastroenterol. 2001 Dec;7(6):816-20. doi: 10.3748/wjg.v7.i6.816.
To explore the role of SF/HGF-Met autocrine and paracrine in metastasis of hepatocellular carcinoma (HCC).
SF/HGF and c-met transcription and protein expression in HCC were examined by RT-PCR and Western Blot in 4 HCC cell lines, including HepG2, Hep3B, SMMC7721 and MHCC-1, the last cell line had a higher potential of metastasis. sf/hgf cDNA was transfected by the method of Lipofectin into SMMC7721. SF/HGF and c-met antibody were used to stimulate and block SF/HGF-c-met signal transduction. Cell morphology, mobility, and proliferation were respectively compared by microscopic observation, wound healing assay and cell growth curve.
HCC malignancy appeared to be relative to its met-SF/HGF expression. In MHCC-1, c-met expression was much stronger than that in other cell lines with lower potential of metastasis and only SF/HGF autocrine existed in MHCC-1. After sf/hgf cDNA transfection or conditioned medium of MHCC-1 stimulation, SMMC7721 changed into elongated morphology, and the abilities of proliferation (P < 0.05) and mobility increased. Such bio-activity could be blocked by c-met antibody (P < 0.05).
The system of SF/HGF-c-met autocrine and paracrine played an important role in development and metastasis potential of HCC. Inhibition of SF/HGF-c-met signal transduction system may reduce the growth and metastasis of HCC.
探讨SF/HGF-Met自分泌和旁分泌在肝细胞癌（HCC）转移中的作用。
采用RT-PCR和Western Blot检测4种肝癌细胞系（包括HepG2、Hep3B、SMMC7721和MHCC-1，最后一种细胞系具有较高的转移潜能）中SF/HGF和c-met的转录及蛋白表达。采用脂质体转染法将sf/hgf cDNA转染至SMMC7721。使用SF/HGF和c-met抗体刺激和阻断SF/HGF-c-met信号转导。通过显微镜观察、伤口愈合试验和细胞生长曲线分别比较细胞形态、迁移能力和增殖能力。
肝癌的恶性程度似乎与其met-SF/HGF表达相关。在MHCC-1中，c-met表达比其他转移潜能较低的细胞系强得多，且MHCC-1中仅存在SF/HGF自分泌。sf/hgf cDNA转染或MHCC-第1条件培养基刺激后，SMMC7721细胞形态变为细长型，增殖能力（P<0.05）和迁移能力增强。这种生物活性可被c-met抗体阻断（P<0.05）。
SF/HGF-c-met自分泌和旁分泌系统在肝癌的发生发展和转移潜能中起重要作用。抑制SF/HGF-c-met信号转导系统可能会降低肝癌的生长和转移。
