脂多糖结合蛋白及其受体CD14在实验性酒精性肝病中的表达
Expression of lipopolysaccharide binding protein and its receptor CD14 in experimental alcoholic liver disease.
作者信息
Zuo G Q, Gong J P, Liu C A, Li S W, Wu X C, Yang K, Li Y
机构信息
Department of Digestive Disease, Second College of Clinical Medicine & the Second Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400010, China.
出版信息
World J Gastroenterol. 2001 Dec;7(6):836-40. doi: 10.3748/wjg.v7.i6.836.
AIM
To evaluate the relationship between the expression of lipopolysaccharides (LPS) binding protein (LBP) and CD14 mRNA and the severity of liver injury in alcohol-fed rats.
METHODS
Twenty Wistar rats were divided into two groups:ethanol-fed group (group E) and control group (group C). Group E was fed with ethanol(5-12 g x kg(-1) x d(-1)) and group C received dextrose instead of ethanol. Rats of the two groups were sacrificed at 4 weeks and 8 weeks. Levels of endotoxin and alanine transaminase (ALT) in blood were measured, and liver pathology was observed under light and electronic microscopy. Expressions of LBP and CD14 mRNA in liver tissues were determined by RT-PCR analysis.
RESULTS
Plasma endotoxin levels were increased more significantly in group E(129+/-21) ng x L(-1) and (187+/-35) ng x L(-1) at 4 and 8 wk than in control rats(48+/-9) ng x L(-1) and (53+/-11) ng x L(-1), respectively (P<0.05). Mean values of plasma ALT levels were (1867+/-250) nkat x L(-1) and (2450+/-367) nkat x L(-1) in Group E. The values were increased more dramatically in ethanol-fed rats than in Group C after 4 and 8 weeks. In liver section from ethanol-fed rats, there were marked pathological changes (steatosis, cell infiltration and necrosis). In ethanol-fed rats, ethanol administration led to a significant increase in LBP and CD14 mRNA levels compared with the control group (P<0.05).
CONCLUSION
Ethanol administration led to a significant increase in endotoxin levels in serum and LBP and CD14 mRNA expressions in liver tissues. The increase of LBP and CD14 mRNA expression might wake the liver more sensitive to endotoxin and liver injury.
目的
评估脂多糖（LPS）结合蛋白（LBP）和CD14 mRNA的表达与酒精喂养大鼠肝损伤严重程度之间的关系。
方法
将20只Wistar大鼠分为两组：乙醇喂养组（E组）和对照组（C组）。E组给予乙醇（5 - 12 g·kg⁻¹·d⁻¹），C组给予葡萄糖而非乙醇。两组大鼠分别在4周和8周时处死。检测血液中内毒素和丙氨酸转氨酶（ALT）水平，并在光镜和电镜下观察肝脏病理变化。通过RT-PCR分析测定肝组织中LBP和CD14 mRNA的表达。
结果
E组在4周和8周时血浆内毒素水平分别为（129±21）ng·L⁻¹和（187±35）ng·L⁻¹，显著高于对照组大鼠的（48±9）ng·L⁻¹和（53±11）ng·L⁻¹（P<0.05）。E组血浆ALT水平的平均值分别为（1867±250）nkat·L⁻¹和（2450±367）nkat·L⁻¹。4周和8周后，乙醇喂养大鼠的ALT值升高幅度比C组更大。在乙醇喂养大鼠的肝脏切片中，有明显的病理变化（脂肪变性、细胞浸润和坏死）。与对照组相比，乙醇喂养大鼠给予乙醇后LBP和CD14 mRNA水平显著升高（P<0.05）。
结论
给予乙醇导致血清内毒素水平以及肝组织中LBP和CD14 mRNA表达显著升高。LBP和CD14 mRNA表达的增加可能使肝脏对内毒素和肝损伤更加敏感。
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