Fu W, Hou J, Wang D
Department of Hematology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Zhonghua Xue Ye Xue Za Zhi. 2001 Nov;22(11):573-6.
To illustrate the role of structure and hypermethylation of p16 gene in the pathogenesis of multiple myeloma(MM).
By using PCR-single strand conformation polymorphisms(PCR-SSCP) and methylation-specific PCR(MSP) techniques, the structure and hypermethylation status of p16 gene in MM cell lines and patients were analysed.
Homozygous deletion of p16 exon 2 was found in KM3 cells. The completely methylated p16 gene and hypermethylation of CPG island were observed in U266, LP1 cell lines and 55.56% of MM patients.
Methylation of p16 gene is important in the pathogenesis of MM and may provide a new drug target for the treatment of MM.
阐明p16基因的结构及高甲基化在多发性骨髓瘤（MM）发病机制中的作用。
采用聚合酶链反应-单链构象多态性（PCR-SSCP）及甲基化特异性PCR（MSP）技术，分析MM细胞系及患者中p16基因的结构及高甲基化状态。
在KM3细胞中发现p16外显子2纯合缺失。在U266、LP1细胞系及55.56%的MM患者中观察到p16基因完全甲基化及CPG岛高甲基化。
p16基因甲基化在MM发病机制中起重要作用，可能为MM治疗提供新的药物靶点。
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