一种用于检测IgG致敏红细胞的酶联免疫吸附测定（ELISA）。

An enzyme linked immunosorbent assay (ELISA) for detecting IgG sensitized erythrocytes.

作者信息

Bruner K W, Kissling C W

出版信息

Transfusion. 1979 Nov-Dec;19(6):773-7. doi: 10.1046/j.1537-2995.1979.19680104108.x.

DOI:10.1046/j.1537-2995.1979.19680104108.x

Abstract

This paper describes an Enzyme Linked Immunosorbent Assay (ELISA) for detecting IgG sensitized erythrocytes utilizing a commercially available anti-human IgG conjugated with alkaline phosphatase. Erythrocyte hemolysis in the assay was minimized by dissolving the p-nitrophenyl phosphate substrate in a carbonate-bicarbonate buffer. Nonspecific absorption of the enzyme conjugate to erythrocytes and glassware was reduced by adding 1% bovine serum albumin to wash solutions. Assay sensitivity was increased with greater concentrations of enzyme conjugate and erythrocytes in the incubation stage. The sensitivity of the described ELISA procedure is approximately equal to that of the standard antiglobulin test. Some possible future applications of ELISA in the blood bank are discussed.

摘要

本文描述了一种酶联免疫吸附测定法（ELISA），用于检测利用与碱性磷酸酶结合的市售抗人IgG来致敏的红细胞。通过将对硝基苯磷酸底物溶解在碳酸盐-碳酸氢盐缓冲液中，可使该测定法中的红细胞溶血降至最低。通过向洗涤液中添加1%牛血清白蛋白，可减少酶结合物对红细胞和玻璃器皿的非特异性吸附。在孵育阶段，随着酶结合物和红细胞浓度的增加，测定灵敏度提高。所描述的ELISA方法的灵敏度与标准抗球蛋白试验的灵敏度大致相当。文中还讨论了ELISA在血库未来一些可能的应用。