烟草叶绿体抗坏血酸过氧化物酶的结晶及初步X射线衍射分析
Crystallization and preliminary X-ray diffraction analysis of chloroplastic ascorbate peroxidase of tobacco plants.
作者信息
Wada Kei, Tada Toshiji, Nakamura Yoshihiro, Yabuta Yukinori, Yoshimura Kazuya, Takeda Toru, Shigeoka Shigeru, Nishimura Keiichiro
机构信息
Research Institute for Advanced Science and Technology, Osaka Prefecture University, Sakai, Osaka 599-8570, Japan.
出版信息
Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):559-61. doi: 10.1107/s0907444902001233. Epub 2002 Feb 21.
The stromal ascorbate peroxidase of tobacco plants was crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 4000 as a precipitant. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 37.2, b = 76.8, c = 98.8 A. The calculated V(M) value based on a monomer in the asymmetric unit is 2.2 A(3)Da(-1). A data set was successfully collected to 1.6 A resolution from a frozen crystal using synchrotron radiation of wavelength 1.0 A at KEK-PF, Japan.
采用悬滴气相扩散法，以聚乙二醇4000作为沉淀剂，使烟草植株的基质抗坏血酸过氧化物酶结晶。晶体属于正交晶系空间群P2(1)2(1)2(1)，晶胞参数a = 37.2、b = 76.8、c = 98.8 Å。基于不对称单元中的一个单体计算得到的V(M)值为2.2 Å(3)Da(-1)。在日本高能加速器研究机构光子工厂（KEK-PF），利用波长为1.0 Å的同步辐射，成功从一个冷冻晶体收集到了分辨率为1.6 Å的数据集。
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