肿瘤坏死因子（TNF）基因内部或附近的多态性并不决定内毒素诱导的TNF产生水平。

Polymorphisms in or near tumour necrosis factor (TNF)-gene do not determine levels of endotoxin-induced TNF production.

作者信息

de Jong B A, Westendorp R G J, Bakker A M, Huizinga T W J

机构信息

Department of Clinical Epidemiology, LUMC, Leiden, The Netherlands.

出版信息

Genes Immun. 2002 Feb;3(1):25-9. doi: 10.1038/sj.gene.6363824.

DOI:10.1038/sj.gene.6363824

PMID:11857057

Abstract

Innate differences in tumour necrosis factor (TNF) production have been associated with susceptibility for and outcome of inflammatory diseases. Several studies have tried to identify whether polymorphisms in or near the TNF gene or other markers on the short arm of chromosome 6 (6p21) are related to differences in TNF production. Data on these associations are conflicting. Therefore, we conducted a study among 129 healthy individuals in which TNF production was determined upon stimulation with endotoxin in whole blood cultures. TNFa microsatellite, TNF single nucleotide polymorphisms at position +489, -238, -308 and -376 typing was performed. The data revealed that alleles of TNFa microsatellite and carriership of TNF polymorphisms were not related to TNF production. We conclude that the genes determing the differences in endotoxin-induced TNF production have not been yet identified.

摘要

肿瘤坏死因子（TNF）产生的固有差异与炎症性疾病的易感性和预后相关。多项研究试图确定TNF基因或6号染色体短臂（6p21）上其他标记附近的多态性是否与TNF产生的差异有关。关于这些关联的数据相互矛盾。因此，我们对129名健康个体进行了一项研究，通过全血培养中内毒素刺激来测定TNF的产生。进行了TNFα微卫星、TNF在+489、-238、-308和-376位点的单核苷酸多态性分型。数据显示，TNFα微卫星的等位基因和TNF多态性的携带情况与TNF产生无关。我们得出结论，尚未确定决定内毒素诱导的TNF产生差异的基因。