McClean Stephen, Robinson Robert C, Shaw Chris, Smyth W Franklin
School of Biomedical Sciences, University of Ulster, Coleraine, County Londonderry BT52 1SA, N. Ireland, UK.
Rapid Commun Mass Spectrom. 2002;16(5):346-54. doi: 10.1002/rcm.583.
The characterisation of selected indole alkaloids in a quadrupole ion trap mass spectrometer is presented. Fragmentation profiles for tryptamine, 5-hydroxytryptamine (5-HT), N'-methyl 5-hydroxytryptamine (N'-methyl 5-HT), N',N'-dimethyl 5-hydroxytryptamine (bufotenine), N',N',N'-trimethyl 5-hydroxytryptamine (5-HTQ), and N',N'-dimethyl 5-methoxytryptamine (5-MeODMT) are presented with proposed structures given for each product ion observed. Such MS(n) experiments can be used to differentiate the isobaric molecular ions of the compounds 5-HTQ (M(+)) and 5-MeODMT (MH(+)). The quantitative determination of certain indole alkaloids in the skin secretions of the Australian Golden Bell frog, Litoria aurea, by LC/ESI-ion trap MS is also presented. The concentrations of 5-HT, N'-methyl 5-HT and 5-HTQ were found to be 2.68, 0.26 and 0.54 microg per mg of skin secretion, respectively.
本文介绍了在四极杆离子阱质谱仪中对选定吲哚生物碱的表征。给出了色胺、5-羟色胺（5-HT）、N'-甲基5-羟色胺（N'-甲基5-HT）、N',N'-二甲基5-羟色胺（蟾蜍色胺）、N',N',N'-三甲基5-羟色胺（5-HTQ）和N',N'-二甲基5-甲氧基色胺（5-MeODMT）的碎裂图谱，并为观察到的每个产物离子给出了推测结构。此类串联质谱（MS(n)）实验可用于区分化合物5-HTQ（M(+)）和5-MeODMT（MH(+)）的等压分子离子。本文还介绍了通过液相色谱/电喷雾离子阱质谱法定量测定澳大利亚金钟蛙（Litoria aurea）皮肤分泌物中某些吲哚生物碱的方法。发现5-HT、N'-甲基5-HT和5-HTQ的浓度分别为每毫克皮肤分泌物2.68、0.26和0.54微克。
