[青霉属细菌5型病毒及其核酸的物理化学特性]
[Physicochemical characteristics of the Penicillium-bacterial virus type 5 and its nucleic acid].
作者信息
Gupalo I D, Surkov V V, Bogush V G, Manykin A A, Parfenov N N
出版信息
Vopr Virusol. 1979 Nov-Dec(6):638-42.
Physico-chemical characteristics of PBV-5 virus and its nucleic acid were studied. Morphologically, the virus is a rectilinear polyhedron 270 A in diameter, without a process. The sedimentation constant of a virus particle is 117S, the buoyant density of the virus in cesium chloride density is 1.437 g/cm3. As shown by qualitative reactions with orcinol and diphenylamine, the virus contains DNA. The viral DNA preparation in CsCl gradient solution showed the buoyant density 1.719 g/cm3. Electron microscope examination of a PBV-5 DNA preparation showed the nucleic acid molecules to be single-stranded rings.
对PBV - 5病毒及其核酸的理化特性进行了研究。从形态学上看，该病毒是直径为270埃的直线型多面体，无突起。病毒粒子的沉降常数为117S，病毒在氯化铯密度中的浮力密度为1.437 g/cm³。用苔黑酚和二苯胺的定性反应表明，该病毒含有DNA。在氯化铯梯度溶液中的病毒DNA制剂显示浮力密度为1.719 g/cm³。对PBV - 5 DNA制剂的电子显微镜检查表明，核酸分子为单链环。
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