Chen Wen-Ya, John Joseph Abraham Christopher, Lin Chen-Hui, Chang Chi-Yao
Institute of Zoology, Academia Sinica, Taiwan, NanKang, Taipei, Republic of China.
Biochem Biophys Res Commun. 2002 Mar 8;291(4):798-805. doi: 10.1006/bbrc.2002.6517.
Metal-responsive transcription factor, MTF-1 is a zinc finger protein, shown to be essential for embryonic development. Homozygous knockout mouse embryos for MTF-1 die in utero at day 14 of gestation, due to liver decay. In the present study, we report the complete nucleotide sequence of cDNA encoding zebrafish MTF-1 and the amino acid sequence similarity with that of mouse, human, fish and Drosophila. The size of the zebrafish MTF-1 cDNA is 3,379 bp and the coding region (1,779 bp) encodes a polypeptide of 593 amino acids. The putative zinc finger and transactivation domains comprised by zebrafish MTF-1 were also determined. The zebrafish MTF-1 shows high identity of 97, 93, 93 and 67% in the DNA binding zinc finger domain and 51, 44, 48 and 20% overall identity with fugu, human, mouse and Drosophila, respectively. RT-PCR results show the maternal expression of MTF-1 transcripts. The pattern of MTF-1 gene expression during embryonic and early larval development was studied by whole-mount in situ hybridization using DIG-labeled anti-sense RNA probe. Stronger and ubiquitous expression was observed during the embryonic stages whereas, specific expression, especially in the neural parts, was observed throughout the stages studied after hatching.
金属反应转录因子MTF-1是一种锌指蛋白,已证明对胚胎发育至关重要。MTF-1基因纯合敲除的小鼠胚胎在妊娠第14天死于子宫内,原因是肝脏衰退。在本研究中,我们报告了编码斑马鱼MTF-1的cDNA的完整核苷酸序列以及与小鼠、人类、鱼类和果蝇的氨基酸序列相似性。斑马鱼MTF-1 cDNA的大小为3379 bp,编码区(1779 bp)编码一个由593个氨基酸组成的多肽。还确定了斑马鱼MTF-1所含的推定锌指和反式激活结构域。斑马鱼MTF-1在DNA结合锌指结构域中与河豚、人类、小鼠和果蝇的同源性分别为97%、93%、93%和67%,总体同源性分别为51%、44%、48%和20%。RT-PCR结果显示MTF-1转录本的母源性表达。使用地高辛标记的反义RNA探针通过整体原位杂交研究了MTF-1基因在胚胎和幼体早期发育过程中的表达模式。在胚胎阶段观察到更强且普遍的表达,而在孵化后的整个研究阶段观察到特异性表达,尤其是在神经部位。