Sumi M G, Mathai A, Reuben S, Sarada C, Radhakrishnan V V
Department of Pathology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram 695011, Kerala State, India.
Clin Diagn Lab Immunol. 2002 Mar;9(2):344-7. doi: 10.1128/cdli.9.2.344-347.2002.
A simple immunocytochemical method was standardized for the direct demonstration of mycobacterial antigen in cerebrospinal fluid (CSF) specimens of patients with tuberculous meningitis (TBM). CSF-cytospin smears were prepared from 22 patients with a clinical diagnosis of TBM and also from an equal number of patients with nontuberculous neurological diseases (disease control). Immunocytological demonstration of mycobacterial antigens in the cytoplasm of monocytoid cells was attempted, by using rabbit immunoglobulin G to Mycobacterium tuberculosis as the primary antibody. Of the 22 CSF-cytospin smears from TBM patients, 16 showed positive immunostaining, while all of the CSF-cytospin smears from the disease control showed negative immunostaining for mycobacterial antigen. The technical aspects of this immunocytological method for the demonstration of mycobacterial antigens are simple, rapid, and reproducible, as well as specific, and therefore can be applied for the early diagnosis of TBM, particularly in patients in whom bacteriological methods did not demonstrate the presence of M. tuberculosis in the CSF.
一种简单的免疫细胞化学方法被标准化，用于直接检测结核性脑膜炎（TBM）患者脑脊液（CSF）标本中的分枝杆菌抗原。从22例临床诊断为TBM的患者以及同等数量的非结核性神经系统疾病患者（疾病对照）中制备CSF细胞涂片。尝试使用兔抗结核分枝杆菌免疫球蛋白G作为一抗，在单核样细胞的细胞质中进行分枝杆菌抗原的免疫细胞化学检测。在22份来自TBM患者的CSF细胞涂片中，16份显示免疫染色阳性，而来自疾病对照的所有CSF细胞涂片对分枝杆菌抗原的免疫染色均为阴性。这种用于检测分枝杆菌抗原的免疫细胞化学方法在技术方面简单、快速、可重复且具有特异性，因此可用于TBM的早期诊断，特别是在细菌学方法未在CSF中检测到结核分枝杆菌的患者中。
