刺槐豆发育中的茎瘤中果胶甲基酯酶转录本的模式。
Patterns of pectin methylesterase transcripts in developing stem nodules of Sesbania rostrata.
作者信息
Lievens Sam, Goormachtig Sofie, Herman Sylvia, Holsters Marcelle
机构信息
Vakgroep Moleculaire Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, Belgium.
出版信息
Mol Plant Microbe Interact. 2002 Feb;15(2):164-8. doi: 10.1094/MPMI.2002.15.2.164.
Differential display was applied to the early stages of the interaction between the tropical legume Sesbania rostrata and its microsymbiont Azorhizobium caulinodans ORS571. An upregulated clone that is similar to pectin methylesterase-encoding genes was isolated (Srpmel). The full-length sequence of Srpme1 was used to localize PME transcripts in situ during S. rostrata stem-nodule development. Several expression patterns were distinguished, hinting at general roles in vascular tissue development and cell division or expansion and at symbiosis-specific functions, such as uninfected cell differentiation.
差异显示技术被应用于热带豆科植物喙荚田菁与其微共生体茎瘤固氮根瘤菌ORS571相互作用的早期阶段。分离出了一个上调克隆，它与编码果胶甲酯酶的基因相似（Srpmel）。利用Srpme1的全长序列在喙荚田菁茎瘤发育过程中对果胶甲酯酶转录本进行原位定位。区分出了几种表达模式，这暗示了其在维管组织发育、细胞分裂或扩展以及共生特异性功能（如未感染细胞分化）中的一般作用。
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