Ren J, Wang S, Li J, Li A
Department of Surgery, Jinling Hospital, 305 East Zhongshan Road, Nanjing 210002, P. R. of China.
Zhonghua Shao Shang Za Zhi. 2000 Aug;16(4):206-9.
To evaluate the effect of early enteral feeding and L-arginine supplementation on mRNA expression of albumin, and some cytokines.
Twenty Wistar rats prepared with gastrostomy 7 days before the experiment were inflicted with 30% TBSA flame and were divided into early enteral feeding group (EF) and L-arginine enriched early enteral feeding group (AEF). All rats in two group starts enteral feeding two hours after burn. Capillary electrophoresis and reverse transcription-polymerase chain reaction (RT-PCR) were used to measure the mRNA of albumin, TNFa, IL-1a, IL-6R and iNOS respectively on 1st, 3rd, 6th and 9th day post burn (PBD). Serum albumin was observed at the same time.
The mRNA expression of albumin on PBD1 was reduced in both group and improved on PBD3, 6, 9. The gene expression of albumin was significantly higher in AEF group than in EF group onPBD3, 9 [(1812 +/- 41) vs (1417 plus minus 43), P < 0.01]. Similarly, serum albumin were very low in two groups on PBD1 and improved on PBD3, 6, 9. Serum albumin in AEF group was significantly higher than in EF group on PBD9 [(31.9 plus minus 2.1) g/L vs (28.1 plus minus 1.3) g/L, P < 0.01]. The mRNA of TNFalpha, IL-1alpha, IL-6R and iNOS were increased on PBD1 and reduced on PBD3, 6, 9. The gene expression of TNFalpha, IL-1alpha in AEF group were significantly lower than EF group on PBD6, while IL-6R differed significantly between AEF group and EF group [(972 +/- 87) vs (2570 +/- 90), P < 0.01] on PBD9. There were no significant difference in iNOS mRNA production after burn in two group.
Early enteral feeding could improve albumin biosynthesis and reduce the gene expression of TNFalpha, IL-1alpha, IL-6R and iNOS. L-arginine-supplemented early enteral feeding could further increase the gene expression of albumin possibly by reducing the expression of TNFalpha, IL-1alpha and IL-6R and did not increase the expression of iNOS.
评估早期肠内营养及补充L-精氨酸对白蛋白及某些细胞因子mRNA表达的影响。
实验前7天制备胃造口的20只Wistar大鼠，给予30%体表面积的火焰烧伤，分为早期肠内营养组（EF）和富含L-精氨酸的早期肠内营养组（AEF）。两组所有大鼠在烧伤后2小时开始肠内营养。分别于烧伤后第1、3、6和9天（PBD），采用毛细管电泳和逆转录-聚合酶链反应（RT-PCR）检测白蛋白、TNFα、IL-1α、IL-6R和iNOS的mRNA。同时观察血清白蛋白水平。
两组在PBD1时白蛋白mRNA表达均降低，在PBD3、6、9时升高。在PBD3、9时，AEF组白蛋白基因表达显著高于EF组[（1812±41）对（1417±43），P<0.01]。同样，两组在PBD1时血清白蛋白水平很低，在PBD3、6、9时升高。在PBD9时，AEF组血清白蛋白显著高于EF组[（31.9±2.1）g/L对（28.1±1.3）g/L，P<0.01]。TNFα、IL-1α、IL-6R和iNOS的mRNA在PBD1时升高，在PBD3、6、9时降低。在PBD6时，AEF组TNFα、IL-1α的基因表达显著低于EF组，而在PBD9时，AEF组和EF组的IL-6R差异显著[（972±87）对（2570±90），P<0.01]。两组烧伤后iNOS mRNA产生无显著差异。
早期肠内营养可改善白蛋白生物合成，降低TNFα、IL-1α、IL-6R和iNOS的基因表达。补充L-精氨酸的早期肠内营养可能通过降低TNFα、IL-1α和IL-6R的表达进一步增加白蛋白的基因表达，且不增加iNOS的表达。
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