26S蛋白酶体在水稻（Oryza sativa L.）分生组织和原基中的空间分布。

Spatial distribution of the 26S proteasome in meristematic tissues and primordia of rice (Oryza sativa L.).

作者信息

Yanagawa Yuki, Kimura Seisuke, Takase Tomomasa, Sakaguchi Kengo, Umeda Masaki, Komamine Atsushi, Tanaka Keiji, Hashimoto Junji, Sato Takahide, Nakagawa Hiroki

机构信息

Department of Bioproduction Science, Faculty of Horticulture, Chiba University, Matsudo, Japan.

出版信息

Planta. 2002 Mar;214(5):703-7. doi: 10.1007/s00425-001-0676-2. Epub 2001 Nov 21.

DOI:10.1007/s00425-001-0676-2

PMID:11882938

Abstract

The 26S proteasome is known to play central roles in the growth of many eukaryotes. However, little is known regarding its distribution in higher plants. Here, we report the spatial distribution pattern of Rpn3 (a regulatory PA700 subunit) and C2 (a subunit of the 20S proteasome) in rice ( Oryza sativa L.) seedlings as determined by in situ hybridization. The transcripts were abundantly co-expressed in the apical and marginal meristems of shoots and roots. Interestingly, these transcripts also accumulated in the leaf and ligule primordia of the shoot apex. Our results suggest that the 26S proteasome is spatially distributed among various tissues and may be involved not only in cell division but also in organ formation in higher plants.

摘要

已知26S蛋白酶体在许多真核生物的生长中发挥核心作用。然而，关于其在高等植物中的分布却知之甚少。在此，我们报告了通过原位杂交确定的水稻（Oryza sativa L.）幼苗中Rpn3（一种调节性PA700亚基）和C2（20S蛋白酶体的一个亚基）的空间分布模式。转录本在茎尖和根尖的顶端及边缘分生组织中大量共表达。有趣的是，这些转录本也在茎尖的叶和叶舌原基中积累。我们的结果表明，26S蛋白酶体在高等植物的各种组织中呈空间分布，可能不仅参与细胞分裂，还参与器官形成。