一种在葡聚糖凝胶LH - 20柱上分离雌激素和植物雌激素的方法。

A method for separating estrogens and phytoestrogens on Sephadex LH-20 columns.

作者信息

Newsome F E, Kitts W D

出版信息

Steroids. 1975 Aug;26(2):215-7. doi: 10.1016/s0039-128x(75)80021-3.

Abstract

A procedure for separating estrogens from phytoestrogens in animal fluids is presented. The use of Sephadex LH-20 in absolute methanol permits a simple separation and allows the measurement of phytoestrogen levels in plasma and urine by the competitive binding assay. This method facilitates a more accurate measure of the hormonal balance in animals with an exogenous input of phytoestrogens.

摘要

本文介绍了一种从动物体液中分离雌激素与植物雌激素的方法。在无水甲醇中使用葡聚糖凝胶LH - 20可实现简单分离，并能通过竞争性结合测定法测量血浆和尿液中的植物雌激素水平。该方法有助于更准确地测定摄入外源性植物雌激素的动物体内的激素平衡。