中国球茎作物中一些卡拉病毒和马铃薯Y病毒属病毒的鉴定。简报。
Characterisation of some carla- and potyviruses from bulb crops in China. Brief report.
作者信息
Chen Jiong, Chen Jien Ping, Adams M J
机构信息
College of Life Sciences, Zhejiang University, Hangzhou, People's Republic of China.
出版信息
Arch Virol. 2002;147(2):419-28. doi: 10.1007/s705-002-8330-y.
Conserved carla- and potyvirus primers were used in RT-PCR to amplify virus fragments from garlic and other bulb crops in China and the fragments were subsequently sequenced and compared in phylogenetic analyses. Garlic plants from Henan, Hubei, Jiangsu, Shangdong and Yunnan provinces all contained at least one isolate each of Garlic latent virus (genus Carlavirus), Onion yellow dwarf virus (OYDV, genus Potyvirus) and Leek yellow stripe virus (LYSV, genus Potyvirus). The complete sequence of a Zhejiang isolate of LYSV was also determined, providing the first complete sequence of this virus. The genome was 10142 nucleotides long excluding the poly(A) tail and had the typical features of the genus Potyvirus, although some of the amino acids surrounding the polyprotein cleavage sites were unusual. Shallot yellow stripe virus (SYSV) was amplified from bunching onion (Allium fistulosum var. caespitosum) in Zhejiang province, providing the first record of SYSV in China. Lily mottle virus was amplified from dragon-teeth lily (Lilium brownii var. viridulum).
在逆转录聚合酶链反应（RT-PCR）中使用保守的卡拉病毒属和马铃薯Y病毒属引物，从中国的大蒜和其他球茎作物中扩增病毒片段，随后对这些片段进行测序，并在系统发育分析中进行比较。来自河南、湖北、江苏、山东和云南省的大蒜植株均至少含有一种大蒜潜隐病毒（卡拉病毒属）、洋葱黄矮病毒（OYDV，马铃薯Y病毒属）和韭菜黄条病毒（LYSV，马铃薯Y病毒属）的分离株。还测定了浙江一株LYSV分离株的完整序列，这是该病毒的首个完整序列。该基因组长度为10142个核苷酸（不包括聚腺苷酸尾），具有马铃薯Y病毒属的典型特征，尽管多聚蛋白切割位点周围的一些氨基酸不同寻常。从浙江省的分葱（Allium fistulosum var. caespitosum）中扩增出了葱黄条病毒（SYSV），这是SYSV在中国的首次报道。从龙牙百合（Lilium brownii var. viridulum）中扩增出了百合斑驳病毒。
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