利用重组p40检测土耳其马血清中博尔纳病病毒（BDV）抗体。简要报告。

Detection of antibodies to Borna disease virus (BDV) in Turkish horse sera using recombinant p40. Brief report.

作者信息

Yilmaz H, Helps C R, Turan N, Uysal A, Harbour D A

机构信息

University of Istanbul, Veterinary Faculty, Department of Microbiology, Avcilar, Turkey.

出版信息

Arch Virol. 2002;147(2):429-35. doi: 10.1007/s705-002-8331-8.

DOI:10.1007/s705-002-8331-8

PMID:11890534

Abstract

The nucleoprotein of Borna disease virus (BDV-p40) was produced in a Baculovirus expression system using sf9 cells. The purity and specificity of the recombinant p40 was confirmed by SDS-PAGE and immunoblotting. The recombinant p40 was used in an ELISA to screen horse sera in Turkey. For this, 323 horses from selected cities in the Marmara region of Turkey were examined clinically and serum was collected from each. All horses were clinically healthy except for a few with wounds on the skin. Antibodies to BDV were detected in the sera of 82 (25%) of 323 horse sera. Six sera were selected that had low, medium or high OD values by ELISA and were analysed by Western blotting. All reacted specifically with p40 at a dilution of 1 in 1000. This is the first report of the detection of Borna disease in Turkey and needs further molecular biological investigations to compare the Turkish strains with those strains detected in Europe.

摘要

博尔纳病病毒核蛋白（BDV-p40）在杆状病毒表达系统中利用sf9细胞产生。通过SDS-PAGE和免疫印迹法确认了重组p40的纯度和特异性。该重组p40用于酶联免疫吸附测定（ELISA）以筛查土耳其的马血清。为此，对来自土耳其马尔马拉地区选定城市的323匹马进行了临床检查，并从每匹马采集了血清。除少数几匹马有皮肤伤口外，所有马临床均健康。在323份马血清中的82份（25%）血清中检测到了抗BDV抗体。选择了6份通过ELISA具有低、中或高OD值的血清，并通过蛋白质印迹法进行分析。所有血清在1:1000稀释度下均与p40发生特异性反应。这是在土耳其检测到博尔纳病的首次报告，需要进一步进行分子生物学研究，以将土耳其毒株与在欧洲检测到的毒株进行比较。