高效基因转移是确定治疗靶点的有效方法：一种功能基因组学方法。
High efficiency gene transfer is an efficient way of defining therapeutic targets: a functional genomics approach.
作者信息
Foxwell B M, Yoshimura S, Bondeson J, Brennan F M, Feldmann M
机构信息
Kennedy Institute of Rheumatology Division, Imperial College School of Medicine, London, UK.
出版信息
Ann Rheum Dis. 2001 Nov;60 Suppl 3(Suppl 3):iii13-7. doi: 10.1136/ard.60.90003.iii13.
Abstract
BACKGROUND
Dendritic cells are the most potent antigen presenting cells and key to many aspects of the immune function. Studying the intracellular signalling mechanism used by dendritic cells would provide an insight into the functioning of these cells and give clues to strategies for immunomodulation.
METHOD
Highly efficient adenoviral infection of dendritic cells for the delivery of transgenes was obtained. These viral vectors were used to introduce IkappaB alpha into dendritic cells for the inhibition of NF-kappaB. This was used to investigate the role of NF-kappaB in dendritic cell function.
RESULTS
By blocking the NF-kappaB function a potent inhibition of the expression of costimulating molecules by dendritic cells with the concomitant loss of T cell stimulating function was demonstrated.
CONCLUSION
The use of adenoviral vectors may be a useful way of studying the role of genes in dendritic cell function.
摘要
背景
树突状细胞是最有效的抗原呈递细胞，也是免疫功能诸多方面的关键。研究树突状细胞所使用的细胞内信号传导机制将有助于深入了解这些细胞的功能，并为免疫调节策略提供线索。
方法
实现了树突状细胞的高效腺病毒感染以递送转基因。这些病毒载体用于将IκBα导入树突状细胞以抑制核因子κB（NF-κB）。这被用于研究NF-κB在树突状细胞功能中的作用。
结果
通过阻断NF-κB功能，证明了树突状细胞对共刺激分子表达的有效抑制以及T细胞刺激功能的随之丧失。
结论
腺病毒载体的使用可能是研究基因在树突状细胞功能中作用的一种有用方法。
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