Wang Joseph
Department of Chemistry, New Mexico State University, Las Cruces, NM 88003, USA.
Electrophoresis. 2002 Mar;23(5):713-8. doi: 10.1002/1522-2683(200203)23:5<713::AID-ELPS713>3.0.CO;2-7.
This article reviews different possibilities for conducting enzymatic assays on microchip platforms, along with potential advantages, limitations, and selected examples of such biochips. Enzyme-based chips combine the analytical power and reagent economy of microfluidic devices with the selectivity and amplification features of biocatalytic reactions. "Lab-on-chip" devices thus allow enzymatic assays to be performed more rapidly, easily, and economically. Such assays usually rely on on-chip mixing and reactions (of the substrates and enzymes) in connection to separations (of the substrates or products). The realization of on-chip enzymatic assays thus requires understanding of how enzymatic reactions behave on a small scale and can be interfaced with separation microchips, and how the microfluidics can be tailored to suit the requirements of particular enzymatic assays. The goal is to obtain sufficient reaction times, without compromising the quality of the analytical separation. The versatility of such on-chip enzymatic assays offers great promise for decentralized testing of clinically or environmentally important substrates.
本文综述了在微芯片平台上进行酶促分析的不同可能性，以及此类生物芯片的潜在优势、局限性和选定示例。基于酶的芯片将微流控设备的分析能力和试剂经济性与生物催化反应的选择性和放大特性结合在一起。因此，“芯片实验室”设备能够更快速、轻松且经济地进行酶促分析。此类分析通常依赖于芯片上（底物与酶的）混合及反应与（底物或产物的）分离相结合。因此，要实现芯片上的酶促分析，就需要了解酶促反应在小尺度下的行为表现以及如何与分离微芯片相连接，还要了解如何定制微流控技术以满足特定酶促分析的要求。目标是在不影响分析分离质量的前提下获得足够的反应时间。此类芯片上酶促分析的多功能性为临床或环境中重要底物的分散式检测带来了巨大希望。
