通过与人类输卵管壶腹细胞共培养改善极低质量人类早期胚胎的发育。
Improved development of very-poor-quality human preembryos by coculture with human fallopian ampullary cells.
作者信息
Weichselbaum Amnon, Paltieli Yoav, Philosoph Ralph, Rivnay Benjamin, Coleman Raymond, Seibel Machelle M, Bar-Ami Shalom
机构信息
Faulkner Institute for Reproductive Medicine, Boston, Massachusetts, USA.
出版信息
J Assist Reprod Genet. 2002 Jan;19(1):7-13. doi: 10.1023/a:1014002404950.
PURPOSE
To determine whether a confluent culture of fallopian ampullary epithelial cells, taken from women at the end of their reproductive life, is capable of rescuing very-poor-quality preembryos from cleavage arrest and/or degeneration.
METHODS
Human preembryos. rejected for transfer or freezing because of very poor quality, and arrested within 24 h of cleavage, were cultured for 5 days in medium alone or over a confluent culture of fallopian ampullary epithelia] cells. Morphological criteria were utilized to assess preembryo degeneration and stage of development.
RESULTS
The described coculture rescued preembryos from degeneration, enhancing development to the blastocyst stage 2.2-fold, compared with cultures in medium alone. Furthermore, fully expanded and hatching blastocysts were observed only under coculture conditions.
CONCLUSIONS
Very-poor-quality human preembryos may be rescued from degeneration, and their growth and development dramatically improved, when cocultured with a confluent culture of fallopian ampullary epithelial cells.
目的
确定取自处于生殖寿命末期女性的输卵管壶腹上皮细胞汇合培养物是否能够挽救质量极差的胚胎，使其免于卵裂停滞和/或退化。
方法
因质量极差而被拒绝移植或冷冻、且在卵裂24小时内停滞的人类胚胎，分别在单独培养基中或在输卵管壶腹上皮细胞汇合培养物上培养5天。利用形态学标准评估胚胎退化情况和发育阶段。
结果
与单独在培养基中培养相比，所述共培养挽救了胚胎免于退化，使发育至囊胚阶段的比例提高了2.2倍。此外，仅在共培养条件下观察到了完全扩张和正在孵化的囊胚。
结论
当与输卵管壶腹上皮细胞汇合培养物共培养时，质量极差的人类胚胎可能会免于退化，其生长和发育会得到显著改善。
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