锥虫线粒体3'末端尿苷酰转移酶（TUTase）：U插入/缺失RNA编辑中的关键酶。
Trypanosome mitochondrial 3' terminal uridylyl transferase (TUTase): the key enzyme in U-insertion/deletion RNA editing.
作者信息
Aphasizhev Ruslan, Sbicego Sandro, Peris Marian, Jang Sei-Heon, Aphasizheva Inna, Simpson Agda M, Rivlin Anatoly, Simpson Larry
机构信息
Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA.
出版信息
Cell. 2002 Mar 8;108(5):637-48. doi: 10.1016/s0092-8674(02)00647-5.
A 3' terminal RNA uridylyltransferase was purified from mitochondria of Leishmania tarentolae and the gene cloned and expressed from this species and from Trypanosoma brucei. The enzyme is specific for 3' U-addition in the presence of Mg(2+). TUTase is present in vivo in at least two stable configurations: one contains a approximately 500 kDa TUTase oligomer and the other a approximately 700 kDa TUTase complex. Anti-TUTase antiserum specifically coprecipitates a small portion of the p45 and p50 RNA ligases and approximately 40% of the guide RNAs. Inhibition of TUTase expression in procyclic T. brucei by RNAi downregulates RNA editing and appears to affect parasite viability.
从热带利什曼原虫线粒体中纯化出一种3'末端RNA尿苷酰转移酶，并克隆了该基因，在该物种以及布氏锥虫中进行表达。该酶在Mg(2+)存在的情况下对3'端添加尿苷具有特异性。TUTase在体内至少以两种稳定形式存在：一种含有约500 kDa的TUTase寡聚体，另一种含有约700 kDa的TUTase复合物。抗TUTase抗血清能特异性共沉淀一小部分p45和p50 RNA连接酶以及约40%的引导RNA。RNA干扰抑制布氏锥虫前循环期TUTase的表达会下调RNA编辑，并似乎影响寄生虫的活力。
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