Aphasizhev Ruslan, Sbicego Sandro, Peris Marian, Jang Sei-Heon, Aphasizheva Inna, Simpson Agda M, Rivlin Anatoly, Simpson Larry
Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Cell. 2002 Mar 8;108(5):637-48. doi: 10.1016/s0092-8674(02)00647-5.
A 3' terminal RNA uridylyltransferase was purified from mitochondria of Leishmania tarentolae and the gene cloned and expressed from this species and from Trypanosoma brucei. The enzyme is specific for 3' U-addition in the presence of Mg(2+). TUTase is present in vivo in at least two stable configurations: one contains a approximately 500 kDa TUTase oligomer and the other a approximately 700 kDa TUTase complex. Anti-TUTase antiserum specifically coprecipitates a small portion of the p45 and p50 RNA ligases and approximately 40% of the guide RNAs. Inhibition of TUTase expression in procyclic T. brucei by RNAi downregulates RNA editing and appears to affect parasite viability.
从热带利什曼原虫线粒体中纯化出一种3'末端RNA尿苷酰转移酶，并克隆了该基因，在该物种以及布氏锥虫中进行表达。该酶在Mg(2+)存在的情况下对3'端添加尿苷具有特异性。TUTase在体内至少以两种稳定形式存在：一种含有约500 kDa的TUTase寡聚体，另一种含有约700 kDa的TUTase复合物。抗TUTase抗血清能特异性共沉淀一小部分p45和p50 RNA连接酶以及约40%的引导RNA。RNA干扰抑制布氏锥虫前循环期TUTase的表达会下调RNA编辑，并似乎影响寄生虫的活力。
