Kuraoka A, Yamanaka I, Miyahara A, Shibata Y, Uemura T
Department of Otorhinolaryngology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan.
Eur Arch Otorhinolaryngol. 1994;251 Suppl 1:S95-9. doi: 10.1007/BF02565230.
We examined protein components of the gap junctions between acinar cells of the parotid, sublingual and submandibular glands of the rat, using type-specific antibodies directed against major gap junction proteins, connexin32 (Cx32) and connexin26 (Cx26). Double-immunofluorescence microscopy revealed that fluorescent spots of both connexins in the parotid and sublingual glands were distributed between the apposed regions of acinar cells. They appeared together, or were co-localized. The intensity of the Cx26-associated fluorescent signals was relatively weak in the submandibular glands compared with the other glands and was absent from some acini. When present, these spots were always co-localized with Cx32 immunoreactive positive spots. The results suggest that Cx32 and Cx26 in rat salivary glands are colocalized within the same gap junctional plaques when simultaneously expressed by the same acinar cells.
我们使用针对主要缝隙连接蛋白连接蛋白32（Cx32）和连接蛋白26（Cx26）的类型特异性抗体，研究了大鼠腮腺、舌下腺和下颌下腺腺泡细胞之间缝隙连接的蛋白质成分。双重免疫荧光显微镜检查显示，腮腺和舌下腺中两种连接蛋白的荧光斑点分布在腺泡细胞的相邻区域之间。它们同时出现，或共定位。与其他腺体相比，下颌下腺中与Cx26相关的荧光信号强度相对较弱，并且在一些腺泡中不存在。当存在时，这些斑点总是与Cx32免疫反应阳性斑点共定位。结果表明，当大鼠唾液腺中的Cx32和Cx26由同一腺泡细胞同时表达时，它们在同一缝隙连接斑块中共定位。
