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一种测定尿酸的机械化酶法(作者译)

[A mechanized enzymic method for the determination of uric acid (author's transl)].

作者信息

Hunziker P, Keller H

出版信息

Z Klin Chem Klin Biochem. 1975 Mar;13(3):89-96.

PMID:1189525
Abstract

A continuous flow method is described for the determination of uric acid in serum using uricase and peroxidase, with omicron-dianisidine as the chromogen. The method can be used with single channel systems and with the multichannel system SMA 12/60 (Technicon). The new method was compared with six other manual and mechanized methods for the dosage of uric acid, and the results were analyzed statistically. The results from all the enzymic methods showed a good correlation, whereas the "chemical oxidation" methods showed systematic deviations.

摘要

描述了一种使用尿酸酶和过氧化物酶,以邻联茴香胺为显色剂测定血清中尿酸的连续流动法。该方法可用于单通道系统和Technicon公司的SMA 12/60多通道系统。将该新方法与其他六种测定尿酸的手动和机械化方法进行了比较,并对结果进行了统计学分析。所有酶法的结果显示出良好的相关性,而“化学氧化”法显示出系统偏差。

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