Shirai H, Sato T, Oka M, Hara T, Mori S
Department of Removable Prosthodontics, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama City 700-8525, Japan.
J Oral Rehabil. 2002 Mar;29(3):287-94. doi: 10.1046/j.1365-2842.2002.00817.x.
The effect of calcium supplementation on the bone dynamics in the hard palate of the molar region (maxilla), mandible and proximal tibia in experimental osteoporotic rats was examined. Ninety ovariectomized (OVX) and 45 sham-OVX Wistar female rats were used in this study. All the rats received surgical operation at 6 weeks of age. Ovariectomized rats were fed on a low calcium diet (0.02%) for 12 weeks post-operation, and then randomly divided into the two following groups. One group was fed on high calcium diet (2.30%) (OVX-HCa) and the other group was remained on the low calcium diet (OVX-LCa). Sham-OVX rats were fed on regular calcium diet (1.15%) during the experimental period (Sham-OVX). Histomorphological analysis was carried out from 12 to 32 weeks post-operation. On undecalcified thin section, bone volume, eroded surface, osteoid surface and bone formation rate were calculated for cortical bone of the maxilla, and for cancellous bone of the mandible and proximal tibia. In the OVX-LCa group, compared with the Sham-OVX group, decrease of the bone volume and increase of the bone resorption and formation parameters were detected throughout the observation periods. In the OVX-HCa group, compared with the OVX-LCa group, increase of the bone volume and temporarily increased parameters of bone formation at 1 week after feeding on high calcium diet were observed in the maxilla, but these changes were not observed in the mandible and proximal tibia. Moreover, the bone resorption and formation parameters in the maxilla, mandible and proximal tibia in the OVX-HCa group became equivalent to the Sham-OVX levels with the passage of time.
研究了补钙对实验性骨质疏松大鼠磨牙区（上颌骨）硬腭、下颌骨和胫骨近端骨动力学的影响。本研究使用了90只去卵巢（OVX）和45只假去卵巢Wistar雌性大鼠。所有大鼠在6周龄时接受手术。去卵巢大鼠术后12周饲喂低钙饮食（0.02%），然后随机分为以下两组。一组饲喂高钙饮食（2.30%）（OVX-HCa），另一组继续饲喂低钙饮食（OVX-LCa）。假去卵巢大鼠在实验期间饲喂常规钙饮食（1.15%）（假去卵巢组）。术后12至32周进行组织形态学分析。在不脱钙薄片上，计算上颌骨皮质骨、下颌骨和胫骨近端松质骨的骨体积、侵蚀表面、类骨质表面和骨形成率。在OVX-LCa组中，与假去卵巢组相比，在整个观察期内检测到骨体积减少，骨吸收和形成参数增加。在OVX-HCa组中，与OVX-LCa组相比，在上颌骨中观察到高钙饮食喂养1周后骨体积增加，骨形成参数暂时增加，但在下颌骨和胫骨近端未观察到这些变化。此外，随着时间的推移，OVX-HCa组上颌骨、下颌骨和胫骨近端的骨吸收和形成参数与假去卵巢组水平相当。
