Urban Nathaniel N, González-Burgos Guillermo, Henze Darrell A, Lewis David A, Barrionuevo German
Department of Neuroscience, University of Pittsburgh, Pittsburgh, PA 15260, USA.
J Physiol. 2002 Mar 15;539(Pt 3):707-12. doi: 10.1113/jphysiol.2001.015024.
We have employed in vitro physiological methods to investigate dopaminergic modulation of excitatory synaptic transmission in monkey prefrontal cortex (PFC) circuits. We show that combined activation of D1-like and D2-like dopamine receptors results in the reduction of extracellular stimulation-evoked isolated EPSCs in layer 3 pyramidal neurons. Using paired recordings from synaptically connected pyramidal neurons we have determined the basic properties of unitary synaptic connections between layer 3 pyramids in the primate PFC and, interestingly, we found that dopamine does not reduce synaptic transmission between nearby pairs of synaptically coupled PFC pyramidal neurons. This input specificity may be a critical aspect of the dopaminergic regulation of recurrent excitatory circuits in the PFC.
我们采用体外生理学方法来研究猴子前额叶皮层(PFC)回路中多巴胺能对兴奋性突触传递的调节作用。我们发现,D1样和D2样多巴胺受体的联合激活会导致第3层锥体神经元中细胞外刺激诱发的孤立兴奋性突触后电流(EPSCs)减少。通过对突触连接的锥体神经元进行配对记录,我们确定了灵长类动物PFC中第3层锥体神经元之间单一突触连接的基本特性,有趣的是,我们发现多巴胺并不会减少相邻的突触耦合PFC锥体神经元对之间的突触传递。这种输入特异性可能是PFC中反复兴奋性回路多巴胺能调节的一个关键方面。